Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR.
Expression profiling of selected microRNA signatures in plasma and tissues of Saudi colorectal cancer patients by qPCR.
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DOI：
10.3892/ol.2015.4068
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发表时间：
2016-02
期刊：
影响因子：
2.9
通讯作者：
Aboul-Soud MA
中科院分区：
文献类型：
--
作者：
Al-Sheikh YA;Ghneim HK;Softa KI;Al-Jobran AA;Al-Obeed O;Mohamed MA;Abdulla M;Aboul-Soud MA
微RNA（miRNAs或miRs）已被提倡作为包括癌症在内的多种疾病状况的潜在稳健且高度稳定的生物标志物。本研究的主要目的是双重的：i）采用逆转录-定量聚合酶链反应，在来自患者的20个结直肠癌（CRC）组织与邻近的无肿瘤粘膜组织的配对研究设计中，分析所选成熟miRNA标签基因（例如miR-145、miR-195、miR-29和miR-92）的表达水平;和ii）检测它们在相同CRC患者血浆中相对于健康对照的年龄匹配血浆的表达水平。与邻近的无肿瘤的粘膜组织相比，在CRC组织中观察到miR-29（2.5）和miR-92（2.6）的统计学显著（P<0.01）增加。CRC血浆样本的分析显示，循环miR-29和miR-92的表达水平显著高于年龄匹配的正常血浆（P<0.01）。相比之下，miR-145和miR-195在CRC组织样品中的平均表达水平相对于正常组织表现出显著（P<0.05）降低。CRC血浆中miR-145和miR-195的平均表达水平显著低于健康对照组（P<0.05）。相对于对照血浆，在II期和IV期CRC患者血浆中观察到四种miRNA基因谱表达水平的明显阶段依赖性变化。总之，结果清楚地反映了组织和血浆中四种miRNA表达水平的相似趋势以及组织和血浆中miRNA水平的正相关性。这些发现可能有助于阐明结直肠癌发生的分子机制，并强调所研究的miRNAs作为结直肠癌新的早期诊断生物标志物的潜力。
MicroRNAs (miRNAs or miRs) have been advocated as potentially robust and highly stable biomarkers of diverse disease conditions including cancer. The primary aim of this study was two-fold: i) to profile the expression levels of selected mature miRNA signature genes, such as miR-145, miR-195, miR-29 and miR-92, in a paired-study design of 20 colorectal cancer (CRC) tissues from patients versus adjacent neoplasm-free mucosal tissues employing reverse transcription-quantitative polymerase chain reaction; and ii) to examine their expression level in the plasma of the same CRC patients in relation to the age-matched plasma of healthy controls. Statistically significant (P<0.01) increases in miR-29 (2.5) and miR-92 (2.6) were observed in CRC tissues compared with adjacent neoplasm-free mucosal tissues. Profiling of CRC plasma samples showed that the expression levels of circulating miR-29 and miR-92 were significantly higher (P<0.01) than in the age-matched normal plasma. By contrast, miR-145 and miR-195 exhibited significant (P<0.05) decreases in their mean expression levels in CRC tissue samples in relation to the normal tissues. The mean expression levels of miR-145 and miR-195 were significantly lower (P<0.05) in CRC plasma than the healthy controls. Distinct stage-dependent changes in the expression level of the four miRNA gene profiles were observed between stages II and IV plasma of CRC patients relative to the control plasma. Taken together, the results clearly reflect a similar trend for the four miRNA expression levels in tissue and plasma as well as the positive correlation in the levels of miRNAs in tissues and plasma. These findings may be useful to clarify the molecular mechanisms underlying colorectal carcinogenesis and to underscore the potential of the investigated miRNAs as novel early diagnostic biomarkers of CRC.