Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis
Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis
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DOI：
10.1038/s44161-022-00138-1
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发表时间：
2022-10-01
期刊：
影响因子：
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通讯作者：
De Vlaminck, Iwijn
中科院分区：
文献类型：
--
作者：
Mantri, Madhav;Hinchman, Meleana M.;De Vlaminck, Iwijn
A significant fraction of sudden death in children and young adults is due to viral myocarditis, an inflammatory disease of the heart. In this study, by using integrated single-cell and spatial transcriptomics, we created a high-resolution, spatially resolved transcriptome map of reovirus-induced myocarditis in neonatal mouse hearts. We assayed hearts collected at three timepoints after infection and studied the temporal, spatial and cellular heterogeneity of host-virus interactions. We further assayed the intestine, the primary site of reovirus infection, to establish a full chronology of molecular events that ultimately lead to myocarditis. We found that inflamed endothelial cells recruit cytotoxic T cells and undergo pyroptosis in the myocarditic tissue. Analyses of spatially restricted gene expression in myocarditic regions and the border zone identified immune-mediated cell-type-specific injury and stress responses. Overall, we observed a complex network of cellular phenotypes and spatially restricted cell-cell interactions associated with reovirus-induced myocarditis in neonatal mice.
