Distribution and Translocation of 141Ce (III) in Horseradish
Distribution and Translocation of 141Ce (III) in Horseradish
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DOI：
10.1093/aob/mcm244
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发表时间：
2007-12-01
期刊：
影响因子：
4.2
通讯作者：
Huang, Xiaohua
中科院分区：
文献类型：
--
作者：
Guo, Xiaoshan;Zhou, Qing;Huang, Xiaohua
背景与目的稀土元素被广泛应用于农业，大量污染环境并进入食品中。研究了Ce-141 （III）在辣根中的分布和转运，以帮助了解稀土元素在植物体内的生化行为和毒性机制。方法应用放射自显影、液体闪烁计数（LSC）和电子显微放射自显影（EMARG）技术研究Ce-141 （III）在辣根中的分布和易位。采用电感耦合等离子体原子发射光谱仪（ICP-AES）分析了Ce-141 （III）和营养元素的含量。结果放射自显影和LSC结果表明，Ce-141 （III）能被辣根吸收，并由叶片转移到叶柄，再转移到根部。Ce-141 （III）在辣根不同部位的含量为：根、叶、茎、叶。辣根对Ce-141 （III）的吸收速率随器官和时间的不同而变化。发育叶片中Ce-141 （III）含量高于成熟叶片。EMARG结果表明Ce-141 （III）可以穿透细胞膜进入叶肉细胞，存在于细胞外和细胞内沉积中。Ce-141 （III）处理降低了辣根中常量营养素的含量。结论Ce-141 （III）能随时间在辣根各器官间吸收转移，且在不同生长阶段分布不同。Ce-141 （III）可以通过外质体和共质体通道或间连丝进入叶肉细胞。Ce-141 （III）会干扰辣根中常量营养素的代谢。
Background and Aims Rare earth elements (REEs) are used in agriculture and a large amount of them contaminate the environment and enter foods. The distribution and translocation of Ce-141 (III) in horseradish was investigated in order to help understand the biochemical behaviour and toxic mechanism of REEs in plants.Methods The distribution and translocation of Ce-141 (III) in horseradish were investigated using autoradiography, liquid scintillation counting (LSC) and electron microscopic autoradiography (EMARG) techniques. The contents of Ce-141 (III) and nutrient elements were analysed using an inductively coupled plasma-atomic emission spectrometer (ICP-AES).Results The results from autoradiography and LSC indicated that Ce-141 (III) could be absorbed by horseradish and transferred from the leaf to the leaf-stalk and then to the root. The content of Ce-141 (III) in different parts of horseradish was as follows: root > leaf-stalk > leaf. The uptake rates of Ce-141 (III) in horseradish changed with the different organs and time. The content of Ce-141 (III) in developing leaves was greater than that in mature leaves. The results from EMARG indicated that Ce-141 (III) could penetrate through the cell membrane and enter the mesophyll cells, being present in both extra- and intra-cellular deposits. The contents of macronutrients in horseradish were decreased by Ce-141 (III) treatment.Conclusions Ce-141 (III) can be absorbed and transferred between organs of horseradish with time, and the distribution was found to be different at different growth stages. Ce-141 (III) can enter the mesophyll cells via apoplast and symplast channels or via plasmodesmata. Ce-141 (III) can disturb the metabolism of macronutrients in horseradish.