Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain
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DOI：
10.2340/00015555-1335
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发表时间：
2012-01-01
影响因子：
3.6
通讯作者：
Smajs, David
中科院分区：
文献类型：
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作者：
Flasarova, Magdalena;Pospisilova, Petra;Smajs, David
从2004年至2010年期间在捷克共和国收集的294名疑似梅毒患者中分离出的415份临床样本进行了梅毒螺旋体DNA检测。标准血清学检测显示，197名患者为梅毒血清阳性，97名患者为梅毒血清阴性。在每个样品中，对polA（TP 0105）、tmpC（TP 0319）、TP 0136、TP 0548和23S rRNA基因进行PCR检测。从91名患者中采集的样本为PCR阳性。梅毒螺旋体DNA分子分型基于TP 0136、TP 0548和23S rRNA基因测序。64例PCR阳性患者的梅毒螺旋体DNA可分型，共检出9种基因型，对大环内酯类抗生素耐药的占37.3%。在39例患者的DNA样本中，进行了疾病控制和预防中心批准的平行密螺旋体分型。阿普和tpr基因的变体似乎独立地与TP 0136、TP 0548和23 S rRNA基因的序列变体联合收割机结合。
A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.