Characterization and specificity of a high titre polyclonal goat anti-murine IL-1 antibody.
Characterization and specificity of a high titre polyclonal goat anti-murine IL-1 antibody.
复制标题
高滴度多克隆山羊抗鼠 IL-1 抗体的表征和特异性。
作者：
M. Scheetz;D. G. Carlson;J. L. Bobbitt;L. Butler;S. Zuckerman
使用源自 LPS 诱导的 P388D1 培养物上清液的 HPLC 纯化的 IL-1 制剂对山羊进行免疫，从而产生了针对鼠 IL-1 特异性的高滴度抗血清。该抗血清在 30,000 倍稀释度下对 IL-1 依赖性胸腺细胞共刺激测定具有 50% 的抑制作用，并且在 100,000 倍稀释度下仍可检测到抗体活性。山羊抗鼠 IL-1 血清对几种鼠巨噬细胞系合成的鼠 IL-1 具有特异性，并且不会中和人、兔或猪（分解代谢蛋白）IL-1。该抗血清还抑制抗原诱导的 D10.G4 辅助 T 细胞系增殖。除了针对 IL-1 的反应外，抗血清还检测到另外三种分子量在 20,000 至 30,000 之间的肽。
The immunization of goats with an HPLC-purified IL-1 preparation derived from the supernatants of LPS-induced P388D1 cultures has resulted in the derivation of a high titre antiserum specific for murine IL-1. This antiserum exhibits a 50% inhibition of an IL-1-dependent thymocyte costimulation assay at a reciprocal dilution of 30,000 and antibody activity is still detected at 100,000-fold dilution. The goat anti-murine IL-1 serum is specific for murine IL-1 synthesized by several murine macrophage cell lines and does not neutralize human, rabbit or porcine (catabolin) IL-1. The antiserum also inhibits antigen-induced proliferation of the D10.G4 helper T-cell line. In addition to the reaction against IL-1, the antiserum also detects three additional peptides with molecular weights between 20,000 and 30,000.