Expression analysis of PMP22/Gas3 in premalignant and malignant pancreatic lesions
Expression analysis of PMP22/Gas3 in premalignant and malignant pancreatic lesions
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DOI：
10.1369/jhc.4a6546.2005
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发表时间：
2005-07-01
影响因子：
3.2
通讯作者：
Friess, H
中科院分区：
文献类型：
--
作者：
Li, JS;Kleeff, J;Friess, H
PMP 22是雪旺细胞的结构蛋白，但它也影响细胞增殖。本研究采用定量RT-PCR和免疫组织化学方法检测了正常胰腺（n=20）、慢性胰腺炎（n= 22）、胰腺导管腺癌（n=31）、导管内乳头状黏液性肿瘤（n=9）、黏液性囊性肿瘤（n=4）、和一组PanIN病变（n=29）。与正常胰腺组织相比，CP（3倍）和PDAC（2.5倍）中的PMP 22 mRNA水平显著较高。PMP 22表达仅限于正常胰腺中的神经，而在CP和PDAC中，PMP 22也在PanIN病变和小百分比的胰腺癌细胞中表达。PMP 22在IPMN和MCNs的肿瘤细胞中弱表达或缺失。PMP 22 mRNA在培养的胰腺癌细胞中以不同的水平存在，并且在8个这些细胞系中的2个中被转化生长因子（TGF）-β 1上调。总之，PMP 22表达存在于CP和PDAC组织中。其在PanIN病变和一些胰腺癌细胞中的体外和体内表达表明PMP 22在从正常胰腺到癌前病变到胰腺癌的肿瘤转化过程中的作用。
PMP22 is a structural protein of Schwann cells, but it also influences cell proliferation. In the present study, quantitative RT-PCR (QRT-PCR) and immunohistochemistry were used to determine PMP22 mRNA levels and to localize PMP22 in the normal pancreas (n=20), chronic pancreatitis (CP) (n=22), pancreatic ductal adenocarcinoma (PDAC) (n=31), intraductal papillary mucinous neoplasms (IPMN) (n=9), mucinous cystic tumors (MCN) (n=4), and in a panel of PanIN lesions (n=29). PMP22 mRNA levels were significantly higher in CP (3-fold) and PDAC (2.5-fold), compared to normal pancreatic tissues. PMP22 expression was restricted to nerves in the normal pancreas, while in CP and PDAC PMP22 was also expressed in PanIN lesions and in a small percentage of pancreatic cancer cells. PMP22 was weak to absent in the tumor cells of IPMNs and MCNs. PMP22 mRNA was present at different levels in cultured pancreatic cancer cells and up-regulated by transforming growth factor (TGF)-beta 1 in 2 of 8 of these cell lines. In conclusion, PMP22 expression is present in both CP and PDAC tissues. Its expression in PanIN lesions and some pancreatic cancer cells in vitro and in vivo suggests a role of PMP22 in the neoplastic transformation process from the normal pancreas to pre-malignant lesions to pancreatic cancer.