Toxicological effects of urban particulate matter on corneal and conjunctival epithelial cells
Toxicological effects of urban particulate matter on corneal and conjunctival epithelial cells
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DOI：
10.1007/s43188-019-00034-0
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发表时间：
2020-02-10
影响因子：
2.3
通讯作者：
Kim, Chan-Sik
中科院分区：
文献类型：
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作者：
Hyun, Soo-Wang;Song, Su Jeong;Kim, Chan-Sik
暴露于城市颗粒物（UPM）是各种眼表疾病（包括干眼综合征）的高危因素。然而，UPM对角膜和结膜上皮损伤的影响尚未完全阐明。在这项研究中，我们研究了UPM暴露在高浓度下的毒理学效应，通过使用体外培养。在暴露于UPM后24 h，观察角膜和结膜上皮细胞的细胞活力、粘蛋白表达和分泌的炎症介质。流式细胞仪检测UPM作用24 h后细胞周期的变化。UPM以剂量依赖性方式降低细胞活力，并增加S期和G2期的细胞数量。UPM暴露减弱了粘蛋白-1的表达，但粘蛋白-4没有。UPM可增加IL-6的释放，减少IL-8的释放。2 '，7'-二氯荧光素二乙酸酯（DCF-DA）的强度在UPM暴露4小时时最高。总之，这些结果表明，UPM通过降低细胞活力、改变细胞周期、破坏粘蛋白和调节炎症介质来引起角膜和结膜上皮细胞的破坏。
Exposure to urban particulate matter (UPM) is a high-risk factor for various ocular surface diseases, including dry eye syndrome. However, the effects of UPM on corneal and conjunctival epithelium damage have not been fully elucidated. In this study, we investigated the toxicological effects of UPM exposure at high concentrations by using in vitro cultures. The cell viability, mucin expression, and the secreted inflammatory mediators of corneal and conjunctival epithelial cells was observed at 24 h after exposure to UPM. The progression of cell cycle was also examined by flow cytometry at 24 h after exposure to UPM. UPM reduced cell viability in a dose-dependent manner and increased cell population in S and G2 phase. The expression of mucin-1 was attenuated by UPM exposure, but that of mucin-4 was not. UPM increased interleukin (IL)-6 release and decreased IL-8 release. The intensity of 2',7'-dichlorofluorescein diacetate (DCF-DA) was highest at 4 h of UPM exposure. In conclusion, these results suggest that UPM causes the disruption of corneal and conjunctival epithelium by decreasing cell viability, altering cell cycle, disrupting mucin, and regulating inflammatory mediators.