Design and construction of a long-term continuous video-EEG monitoring unit for simultaneous recording of multiple small animals
Design and construction of a long-term continuous video-EEG monitoring unit for simultaneous recording of multiple small animals
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DOI：
10.1016/s1385-299x(97)00033-0
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发表时间：
1997-12-01
期刊：
影响因子：
--
通讯作者：
Chen, ZF
中科院分区：
文献类型：
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作者：
Bertram, EH;Williamson, JM;Chen, ZF
近年来，已经描述了几种新的人类边缘/内侧颞叶癫痫大鼠模型[1，2，4 - 7，11，15 -17]。与早期的模型（如点燃模型）不同，这些新模型的特征是癫痫发作以随机间隔自发发生。虽然癫痫发作的自发性使这些模型更像人类癫痫，但通过直接观察记录这些癫痫发作是非常低效的，并且亚行为电图癫痫发作可能会被遗漏。已经使用了连续纸EEG和视频记录[5- 7，15]：但这些技术是资源密集型的。长期纸记录所需的缓慢纸速度限制了区分真正癫痫发作活动和电伪影的能力。在仅快速查看视频记录时，可能会错过微妙的行为癫痫发作[16]。已经使用了便携式盒式EEG记录[3]，但系统需要昂贵的专有硬件，并且系统的记录通道有限（8-16）。为了提高模型的实用性，我们开发了一个长期的脑电图/视频监测系统，以检测电图癫痫发作和记录他们的行为伴随。该系统基于市售组件，包括最初为人类癫痫监测而开发的计算机化EEG癫痫检测系统[8，9，13]。癫痫发作被可靠地检测和数据减少，使24小时的记录可以在30-90分钟内进行审查。虽然计算机程序是准确的，必须特别小心，在系统的设计和建设，以减少来源的电伪影，可能会导致错误的检测时，多个动物同时记录在一个单一的脑电图机。在数据审查期间，有必要通过关键EEG模式区分动物活动诱导的电伪影与真实癫痫发作活动。癫痫发作检测程序无法检测到某些癫痫发作模式（小于3 Hz，低振幅），但该系统对典型的边缘系统癫痫发作非常有效，可能对失神癫痫的动物模型有用[12，14]。它还可用作连续或间歇性的EEG/生理记录设备，用于检查动物自发行为和EEG相关性（例如睡眠/觉醒周期、学习和记忆任务）的实验。(C)1997年Elsevier Science B.V.
In recent years several new rat models of human limbic/mesial temporal lobe epilepsy have been described [1,2,4-7,11,15-17]. Unlike earlier models such as kindling in which the seizures are induced by an exogenous stimulus, these new models are characterized by seizures that occur spontaneously at random intervals. Although the spontaneity of the seizures makes these models more like human epilepsy, documentation of these seizures by direct observation is highly inefficient, and subbehavioral electrographic seizures could be missed. Continuous paper EEG and video recording have been used [5-7,15]: but these techniques are resource intensive. The slow paper speed required by long-term paper recordings limits the ability to differentiate between true seizure activity and electrical artifact. Subtle behavioral seizures are likely to be missed during rapid review of video recordings alone [16]. Ambulatory cassette EEG recordings have been used [3], but the systems require expensive proprietary hardware, and the systems have limited channels for recording (8-16). To improve the utility of the models, we developed a long-term EEG/video monitoring system to detect the electrographic seizures and document their behavioral accompaniment. The system is based on commercially available components, including a computerized EEG seizure detection system that was initially developed for human seizure monitoring [8,9,13]. Seizures are reliably detected and the data are reduced so that 24 h of recording can be reviewed in 30-90 min. Although the computer program is accurate, special care must be taken in system design and construction to reduce sources of electrical artifact that can cause false detections when multiple animals are recorded simultaneously on a single EEG machine. During data review it is necessary to differentiate between electrical artifact induced by animal activity from true seizure activity by key EEG patterns. Certain seizure patterns (less than 3 Hz, low amplitude) will not be detected by the seizure detection program, but the system is highly effective for typical limbic seizures and may be useful for the animal models of absence epilepsy [12,14]. It can also be used as a continuous or intermittent EEG/physiological recording device for experiments that examine animals' spontaneous behavior and the EEG correlate (e.g. sleep/wake cycles, learning and memory tasks). (C) 1997 Elsevier Science B.V.