Selenium Doped Graphene Quantum Dots as an Ultrasensitive Redox Fluorescent Switch
Selenium Doped Graphene Quantum Dots as an Ultrasensitive Redox Fluorescent Switch
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DOI：
10.1021/acs.chemmater.5b00112
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发表时间：
2015-03
影响因子：
8.6
通讯作者：
Siwei Yang;Jing Sun;P. He;Xinxia Deng;Zhongyang Wang;Chenyao Hu;Guqiao Ding;Xiaoming Xie
中科院分区：
文献类型：
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作者：
Siwei Yang;Jing Sun;P. He;Xinxia Deng;Zhongyang Wang;Chenyao Hu;Guqiao Ding;Xiaoming Xie
基于硒掺杂石墨烯量子点（Se-GQDs），设计了一种新的可逆荧光开关，用于氧化性羟基自由基（·OH）和还原性谷胱甘肽（GSH）的检测。Se-GQD具有1-3个原子层的厚度，1-5 nm的横向尺寸，0.29的量子产率，以及3.44 ns的光致发光寿命，这确保了荧光开关的高选择性和稳定性。由于C-Se基团的可逆氧化和Se-Se基团的可逆还原，Se-GQDs的荧光分别被·OH和GSH可逆地猝灭和恢复。这种全新的基于GQD的荧光开关在水溶液和活的HeLa细胞中测试时都能快速响应。特别是，·OH的检测限仅为0.3 nM，远低于有机染料制成的开关。
A new reversible fluorescent switch for the detection of oxidative hydroxyl radical (•OH) and reductive glutathione (GSH) was designed based on the use of selenium doped graphene quantum dots (Se-GQDs). The Se-GQDs have a thickness of 1–3 atomic layers, a lateral size of 1–5 nm, a quantum yield of 0.29, and a photoluminescence lifetime of 3.44 ns, which ensured a high selectivity and stability for the fluorescent switch. The fluorescence of Se-GQDs was reversibly quenched and recovered by •OH and GSH, respectively, because of the reversible oxidation of C–Se groups and reduction of Se–Se groups. This brand-new GQD-based fluorescent switch gave a rapid response when tested in both aqueous solutions and living HeLa cells. In particular, the detection limit for •OH was only 0.3 nM, which was much lower than that in switches made from organic dyes.