Altered expression of adenosine A1 and A2A receptors in the carotid body and nucleus tractus solitarius of adult male and female rats following neonatal caffeine treatment
Altered expression of adenosine A1 and A2A receptors in the carotid body and nucleus tractus solitarius of adult male and female rats following neonatal caffeine treatment
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DOI：
10.1016/j.brainres.2009.06.064
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发表时间：
2009-09-01
期刊：
影响因子：
2.9
通讯作者：
Kinkead, Richard
中科院分区：
文献类型：
--
作者：
Bairam, Aida;Joseph, Vincent;Kinkead, Richard
新生咖啡因治疗（腺苷受体拮抗剂，15 mg/kg/天，出生后第 3 天至 12 天）会影响成年雄性大鼠的呼吸模式，但不会影响雌性大鼠，如缺氧反应早期呼吸频率增加和反应后期潮气量增加所示。在这里，我们测试了以下假设：这些变化与化学反射途径中腺苷受体的表达改变相关。颈动脉体、孤束核和颈上神经节从 3 个月大的雄性和雌性大鼠中收集，这些大鼠要么是幼稚的（在新生儿期未进行操作），要么是在出生后 3 天至 12 天之间通过灌胃法用咖啡因 (NCT) 或水 (NWT) 处理。蛋白质印迹分析用于评估腺苷 A(1) 和 A(2A) 受体以及酪氨酸羟化酶（多巴胺合成的限速酶）的表达。在雄性大鼠中，NCT 颈动脉体中 A(2A) 受体水平增加了 37%，酪氨酸羟化酶水平降低了 17%（p
Neonatal caffeine treatment (adenosine receptor antagonist, 15 mg/kg/day, between postnatal days 3 and 12) affects respiratory patterns in adult male but not female rats as shown by an increase in the respiratory frequency in the early phase of response to hypoxia and an increase in the tidal volume in the late phase of response. Here, we tested the hypothesis that these changes are correlated with modified expression of adenosine receptors in the chemoreflex pathway. Carotid bodies, nucleus tractus solitarii, and superior cervical ganglia were collected from 3-month-old male and female rats that were either naive (not manipulated during the neonatal period) or treated with caffeine (NCT) or water (NWT) between postnatal days 3 and 12 by gavage. Western blot analysis was used to assess the expression of adenosine A(1) and A(2A) receptors and tyrosine hydroxylase, the rate-limiting enzyme for dopamine synthesis. In male rats, there was a 37% increase in the level of A(2A) receptor and a 17% decrease in tyrosine hydroxylase in the carotid body of NCT (p