Gap-junctional coupling between neurogliaform cells and various interneuron types in the neocortex
Gap-junctional coupling between neurogliaform cells and various interneuron types in the neocortex
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DOI：
10.1523/jneurosci.1431-05.2005
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发表时间：
2005-07-06
影响因子：
5.3
通讯作者：
Tamás, G
中科院分区：
文献类型：
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作者：
Simon, A;Oláh, S;Tamás, G
电突触有助于神经元网络中同步活动的产生。通过突触后GABA（A）受体起作用的几种类型的皮质GABA能神经元也与同类中间神经元形成电突触，这表明通过间隙连接的同步化可能仅限于同质中间神经元群体。神经胶质样细胞在皮质锥体细胞中引起GABA（A）和GABA（B）受体介导的突触后反应，但尚不清楚神经胶质样细胞是否参与电耦合连接的网络。我们记录了大鼠躯体感觉皮层第2层和第3层（出生后第20-35天）的皮层神经元对、三联体和四联体。神经胶质样细胞诱发锥体细胞上的慢IPSPs也触发中间神经元上的发散电耦合电位。神经胶质细胞电耦合到其他神经胶质细胞，篮状细胞，定期尖峰的非锥体细胞，一个axoaxonic细胞，和各种未分类的interneurons显示不同的放电模式和形态。电相互作用介导的一个或两个电子显微镜验证连接体树突状结构域的耦合细胞的间隙连接。我们的研究结果表明，神经胶质细胞在皮层回路中有一个独特的位置。神经胶质样细胞除了引起GABAA和GABA（B）受体介导的对锥体细胞的抑制外，还建立电突触并连接由间隙连接形成的多个网络，所述间隙连接限于特定类别的中间神经元。广泛的电连接可能使神经胶质细胞能够监测作用于靶细胞不同区域的GABA（A）受体的不同中间神经元的活动。
Electrical synapses contribute to the generation of synchronous activity in neuronal networks. Several types of cortical GABAergic neurons acting via postsynaptic GABA(A) receptors also form electrical synapses with interneurons of the same class, suggesting that synchronization through gap junctions could be limited to homogenous interneuron populations. Neurogliaform cells elicit combined GABA(A) and GABA(B) receptor-mediated postsynaptic responses in cortical pyramidal cells, but it is not clear whether neurogliaform cells are involved in networks linked by electrical coupling. We recorded from pairs, triplets, and quadruplets of cortical neurons in layers 2 and 3 of rat somatosensory cortex (postnatal day 20-35). Neurogliaform cells eliciting slow IPSPs on pyramidal cells also triggered divergent electrical coupling potentials on interneurons. Neurogliaform cells were electrically coupled to other neurogliaform cells, basket cells, regular-spiking nonpyramidal cells, to an axoaxonic cell, and to various unclassified interneurons showing diverse firing patterns and morphology. Electrical interactions were mediated by one or two electron microscopically verified gap junctions linking the somatodendritic domain of the coupled cells. Our results suggest that neurogliaform cells have a unique position in the cortical circuit. Apart from eliciting combined GABAA and GABA(B) receptor-mediated inhibition on pyramidal cells, neurogliaform cells establish electrical synapses and link multiple networks formed by gap junctions restricted to a particular class of interneuron. Widespread electrical connections might enable neurogliaform cells to monitor the activity of different interneurons acting on GABA(A) receptors at various regions of target cells.