Immediate Hypothermia Is Not Neuroprotective After Severe Hypoxia-Ischemia and Is Deleterious When Delayed by 12 Hours in Neonatal Rats
Immediate Hypothermia Is Not Neuroprotective After Severe Hypoxia-Ischemia and Is Deleterious When Delayed by 12 Hours in Neonatal Rats
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DOI：
10.1161/strokeaha.112.674481
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发表时间：
2012-12-01
期刊：
影响因子：
8.3
通讯作者：
Thoresen, Marianne
中科院分区：
文献类型：
--
作者：
Sabir, Hemmen;Scull-Brown, Emma;Thoresen, Marianne
背景和目的——建议在出生后 6 小时内开始治疗新生儿缺氧缺血性脑病的低温 (HT)。有一些临床证据表明HT对中度缺氧缺血性脑病比对重度缺氧缺血性脑病更有效，但尚不清楚延迟HT超过6小时是否有效甚至有害。 方法-17只7日龄大鼠幼仔接受中度单侧缺氧缺血。幼犬被随机分配接受 5 小时的常温 (NT) 或 HT，在 90 分钟缺氧期后立即、3 小时、6 小时或 12 小时开始。 150 只 7 日龄幼鼠经历持续 150 分钟的严重缺氧缺血，随后进行与上述相同的分组设计。存活1周后测量左/右半球的相对面积损失。结果-在中度NT组中，左半球的平均面积损失为40.5%。立即 HT 后面积损失显着降低至 24.8%（P
Background and Purpose-Hypothermia (HT) for neonatal hypoxic-ischemic encephalopathy is advised to start within the first 6 hours after birth. There is some clinical evidence that HT is more effective against moderate than against severe -hypoxic-ischemic encephalopathy, but it is unknown whether delayed HT beyond 6 hours is effective or even injurious.Methods-One-hundred seven 7-day-old rat pups underwent unilateral hypoxia-ischemia of moderate severity. Pups were randomized to receive 5 hours of normothermia (NT) or HT starting immediately, 3 hours, 6 hours, or 12 hours after the 90-minute hypoxic period. One-hundred five 7-day-old rat pups underwent severe hypoxia-ischemia lasting 150 minutes, followed by the same group design as mentioned. Relative area loss of the left/right hemisphere was measured after 1 week of survival.Results-In the moderate NT group, the mean area loss of the left hemisphere was 40.5%. The area loss was significantly decreased to 24.8% with immediate HT (P