16-Mer ferritin-like protein templated gold nanoclusters for bioimaging detection of methylmercury in the brain of living mice
16-Mer ferritin-like protein templated gold nanoclusters for bioimaging detection of methylmercury in the brain of living mice
复制标题
16-Mer 铁蛋白样蛋白模板金纳米簇用于活体小鼠大脑中甲基汞的生物成像检测
DOI：
10.1016/j.aca.2020.06.055
复制
发表时间：
2020-08-22
影响因子：
6.2
通讯作者：
Zhao, Guanghua
中科院分区：
文献类型：
--
作者：
Lv, Chenyan;Yin, Shuhua;Zhao, Guanghua
甲基汞（MeHg+）是毒性最强的神经毒素之一，主要蓄积在脑内，因此脑内MeHg+的活体成像检测具有重要意义。在此，我们报道了一种用于脑中MeHg+检测的光致发光纳米传感器，其通过整合金纳米簇（Au NCs）的生物成像、被MeHg+猝灭的Au NCs的荧光以及我们最近构建的16-mer壳状蛋白（7A）的脑靶向特征。首先，首次采用一种简便的绿色方法在水中制备了以7A为模板的Au纳米粒子（7A-Au NCs），其荧光（~ 650 nm）在体外可被MeHg+以剂量依赖的方式猝灭。第二，所制备的7A-Au NCs不仅适用于BBB内皮细胞的生物成像，而且是脑特异性方式的生物成像MeHg+检测的有效探针。这些发现为在活体大脑中检测甲基汞+打开了一扇大门。(C)2020 Elsevier B. V.保留所有权利。
Methylmercury (MeHg+) as one of the most potent neurotoxins is mainly accumulated in brain, so in vivo imaging detection of MeHg+ in brain is of crucial importance. Herein, we reported a photoluminescent nanosensor for MeHg+ detection in brain by integrating the bioimaging of gold nanoclusters (Au NCs), the fluorescence of Au NCs quenched by MeHg+, and the brain targeting feature of our recently constructed 16-mer shell-like protein (7A). First, Au NCs with 7A as a biotemplate (7A-Au NCs) by a facile and green method in water are fabricated for the first time, the fluorescence of which (-650 nm) can be quenched by MeHg+ in a dose-dependent manner in vitro. Second, the as-prepared 7A-Au NCs are not only suitable for bioimaging of BBB endothelial cells, but also are an effective probe for bioimaging MeHg+ detection in a brain-specific manner. These findings open a door for MeHg+ detection in the brain of living subjects. (C) 2020 Elsevier B.V. All rights reserved.