ANTIBODY-RESPONSE OF SCHISTOSOMA-MANSONI-INFECTED HUMAN-SUBJECTS TO THE RECOMBINANT P-28-GLUTATHIONE-S-TRANSFERASE AND TO SYNTHETIC PEPTIDES
ANTIBODY-RESPONSE OF SCHISTOSOMA-MANSONI-INFECTED HUMAN-SUBJECTS TO THE RECOMBINANT P-28-GLUTATHIONE-S-TRANSFERASE AND TO SYNTHETIC PEPTIDES
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DOI：
10.1128/jcm.28.9.1918-1924.1990
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发表时间：
1990-09-01
影响因子：
9.4
通讯作者：
CAPRON, A
中科院分区：
文献类型：
--
作者：
AURIAULT, C;GRASMASSE, H;CAPRON, A
曼氏血吸虫28千道尔顿抗原曾被认为是潜在疫苗的重要基础。P28重组分子及其主要序列衍生的三个肽，即24-43、115-131和140-153肽，已被用于评估肯尼亚学龄儿童化疗后对再感染易感或耐药的体液反应。我们在这里报道，P28分子和研究的两个肽（肽115-131和140-153）可以用于检测特异性免疫球蛋白G (IgG)， IgE和IgA抗体。此外，易感人群的IgG4应答显著大于耐药组，而两组人群的总IgG抗p28抗体无显著差异。这表明IgG4可能在易感患者缺乏免疫力中发挥作用。观察到强烈的IgG3反应仅限于140-153肽，但在抗性和易感群体之间没有区别。相反，在耐药人群的血清中，IgA对140-153肽表位(s)的反应明显增加。综上所述，这些结果表明，P28抗原和选择的三种肽中的两种可以提供疾病发展或以P28为免疫原的疫苗接种效率的预测信息。
The 28-kilodalton antigen of Schistosoma mansoni has been previously described as having importance as the basis for a potential vaccine. The P28 recombinant molecule and three peptides derived from its primary sequence, namely the 24-43, 115-131, and 140-153 peptides, have been tested to evaluate the humoral responses of Kenyan school children previously classified as susceptible or resistant to reinfection after chemotherapy. We report here that the P28 molecule and two of the peptides studied (peptides 115-131 and 140-153) can be used for detecting specific immunoglobulin G (IgG), IgE, and IgA antibodies. Moreover, the IgG4 response of the susceptible population was significantly greater than that of the resistant group, whereas no differences between the two populations were noticed in total IgG anti-P28 antibodies. This suggested that IgG4 could play a role in the lack of immunity of susceptible patients. A strong IgG3 response restricted to the 140-153 peptide was observed but did not discriminate between the resistant and susceptible populations. In contrast, a marked increase in the IgA response to the 140-153 peptide epitope(s) in sera of the resistant population was noticed. Taken together, these results suggest that the P28 antigen and two of the three peptides selected could give predictive information about the development of the disease or the efficiency of vaccination with P28 as the immunogen.