Plasma polymerized non-fouling thin films for DNA immobilization
Plasma polymerized non-fouling thin films for DNA immobilization
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DOI：
10.1016/j.bios.2009.07.033
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发表时间：
2009-10-15
影响因子：
12.6
通讯作者：
Foerch, Renate
中科院分区：
文献类型：
--
作者：
Chu, Li-Qiang;Knoll, Wolfgang;Foerch, Renate
由于DNA传感器在传染病诊断、食品质量控制、环境监测等领域具有广泛的应用前景，因此，DNA传感器的研究一直是人们关注的热点。大多数DNA传感器涉及单链寡核苷酸的固定，所谓的DNA探针，到传感器表面。在这里，我们报告了一种新的方法，用于DNA探针固定使用链霉亲和素-生物素组件加上无污染的薄膜。二（乙二醇）单乙烯基醚（ppEO 2）的脉冲等离子体聚合将产生无污染的薄膜，其用于固定链霉亲和素。通过仔细控制ppEO（2）膜的厚度和化学性质，包埋的链霉亲和素能够结合生物素化的寡核苷酸。所得的DNA传感器对BSA和纤维蛋白原的吸附表现出良好的抵抗力，并被用来区分不同的DNA序列从含蛋白质的样品溶液中，所看到的表面等离子体增强荧光光谱（SPFS）。这一结果表明，本传感器是非常有前途的检测的DNA序列从复杂的解决方案。(C)2009 Elsevier B. V.保留所有权利。
Development of DNA sensors has been an issue of great interest, owing to their potential applications in different fields, such as the diagnosis of infectious diseases, food quality control, or for environmental monitoring. Most DNA sensors involved the immobilization of single-stranded oligonucleotides, so-called DNA probes, onto the sensor surfaces. Here we report on a new approach for DNA probe immobilization using the streptavidin-biotin assembly coupled with a non-fouling thin film. Pulsed plasma polymerization of di(ethylene glycol) monovinyl ether (ppEO2) will result in non-fouling thin films, which are employed to immobilize streptavidin. By careful control of the thickness and the chemistry of ppEO(2) films, the embedded streptavidin are able to bind the biotinylated oligonucleotides. The resulting DNA sensors show good resistance towards adsorption of both BSA and fibrinogen, and are employed to discriminate different DNA sequences from protein-containing sample solutions, as seen by surface plasmon enhanced fluorescence spectroscopy (SPFS). This result suggests that the present sensor is very promising for the detection of a DNA sequence from a complex solution. (C) 2009 Elsevier B.V. All rights reserved.