E3 ubiquitin ligase gene CMPG1-V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)
E3 ubiquitin ligase gene CMPG1-V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)
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Haynaldia villosa L. 的 E3 泛素连接酶基因 CMPG1-V 有助于普通小麦 (Triticum aestivum L.) 的白粉病抗性
DOI：
10.1111/tpj.12966
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发表时间：
2015-10-01
期刊：
影响因子：
7.2
通讯作者：
Wang, Xiue
中科院分区：
文献类型：
--
作者：
Zhu, Yanfei;Li, Yingbo;Wang, Xiue
白粉病是小麦上最具毁灭性的真菌病害之一。一种二倍体小麦近缘种簇毛麦，高抗白粉病，其抗性遗传资源如Pm21位点已广泛应用于小麦育种。在这里，我们报告的克隆H.villosa的抗性基因，命名为CMPG 1-V，它编码一个U-box E3泛素连接酶。接种小麦白粉病菌（Blumeria graminis f.）菌株（Bgt）的表达，并且Pm21的存在对于其快速诱导表达是必需的。CMPG 1-V具有保守的E3连接酶的关键残基，并且在体外和体内都具有E3连接酶活性。CMPG 1-V定位于细胞核、内质网、质膜和部分位于trans-Golgi网络/早期内体囊泡中。过量表达CMPG 1-V的转基因小麦在苗期和成株期表现出广谱抗白粉病性的提高，与水杨酸响应基因的表达增加、H2 O2积累和Bgt侵染部位细胞壁蛋白交联有关，水杨酸、脱落酸和H2 O2处理均提高了CMPG 1-V在簇毛麦中的表达。这些结果表明E3连接酶参与了小麦对Bgt真菌的防御反应，特别是广谱抗病性，并表明反应性氧化物质和植物激素途径与CMPG 1-V介导的白粉病抗性有关。
Powdery mildew is one of the most devastating wheat fungal diseases. A diploid wheat relative, Haynaldia villosa L., is highly resistant to powdery mildew, and its genetic resource of resistances, such as the Pm21 locus, is now widely used in wheat breeding. Here we report the cloning of a resistance gene from H.villosa, designated CMPG1-V, that encodes a U-box E3 ubiquitin ligase. Expression of the CMPG1-V gene was induced in the leaf and stem of H.villosa upon inoculation with Blumeria graminis f. sp. tritici (Bgt) fungus, and the presence of Pm21 is essential for its rapid induction of expression. CMPG1-V has conserved key residues for E3 ligase, and possesses E3 ligase activity in vitro and in vivo. CMPG1-V is localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans-Golgi network/early endosome vesicles. Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid-responsive genes, H2O2 accumulation, and cell-wall protein cross-linking at the Bgt infection sites, and the expression of CMPG1-V in H.villosa was increased when treated with salicylic acid, abscisic acid and H2O2. These results indicate the involvement of E3 ligase in defense responses to Bgt fungus in wheat, particularly in broad-spectrum disease resistance, and suggest association of reactive oxidative species and the phytohormone pathway with CMPG1-V-mediated powdery mildew resistance.