Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome.
Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome.
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DOI：
10.1155/2013/367956
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发表时间：
2013
影响因子：
2.8
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Boqun X;Xiaonan D;Yugui C;Lingling G;Xue D;Gao C;Feiyang D;Jiayin L;Gao L;Li M;Zhang Y;Ma X
背景资料。我们先前利用基因芯片技术发现SET基因在多囊卵巢中的表达上调。提示SET可能是参与多囊卵巢综合征(PCOS)病理生理过程的一个有吸引力的候选调节因子。本研究对SET蛋白在人多囊卵巢和正常卵巢中的表达和细胞定位进行了研究。方法。在变性手术和手术治疗过程中收集卵巢组织，包括6个正常卵巢和6个多囊卵巢，并签署同意书。免疫组织化学方法观察SET蛋白在细胞内的定位。Western Blot分析SET蛋白的表达水平。结果。在PCOS卵巢组织和正常卵巢组织中，SET蛋白主要在卵泡膜细胞和卵母细胞中表达。SET蛋白在多囊卵巢中的表达水平是正常卵巢的3倍(P<；0.05)。结论。SET在多囊卵巢中的表达高于正常卵巢。SET结合其在膜细胞中的定位，可能参与调节卵巢雄激素的生物合成和PCOS高雄激素血症的病理生理过程。
Background. We previously found that expression of SET gene was up-regulated in polycystic ovaries by using microarray. It suggested that SET may be an attractive candidate regulator involved in the pathophysiology of polycystic ovary syndrome (PCOS). In this study, expression and cellular localization of SET protein were investigated in human polycystic and normal ovaries. Method. Ovarian tissues, six normal ovaries and six polycystic ovaries, were collected during transsexual operation and surgical treatment with the signed consent form. The cellular localization of SET protein was observed by immunohistochemistry. The expression levels of SET protein were analyzed by Western Blot. Result. SET protein was expressed predominantly in the theca cells and oocytes of human ovarian follicles in both PCOS ovarian tissues and normal ovarian tissues. The level of SET protein expression in polycystic ovaries was triple higher than that in normal ovaries (P < 0.05). Conclusion. SET was overexpressed in polycystic ovaries more than that in normal ovaries. Combined with its localization in theca cells, SET may participate in regulating ovarian androgen biosynthesis and the pathophysiology of hyperandrogenism in PCOS.
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影响因子：
5.8
作者：
Nelson, VL;Qin, KN;McAllister, JM
通讯作者：
McAllister, JM
影响因子：
--
作者：
Nelson, VL;Legro, RS;McAllister, JM
通讯作者：
McAllister, JM
影响因子：
5.8
作者：
Wickenheisser, JK;Nelson-DeGrave, VL;McAllister, JM
通讯作者：
McAllister, JM
影响因子：
--
作者：
Compagnone, NA;Zhang, P;Mellon, SH
通讯作者：
Mellon, SH
影响因子：
64.5
作者：
Seo, SB;McNamara, P;Chakravarti, D
通讯作者：
Chakravarti, D