Selective Detection of 5-Formyl-2′-deoxycytidine in DNA Using a Fluorogenic Hydroxylamine Reagent
Selective Detection of 5-Formyl-2′-deoxycytidine in DNA Using a Fluorogenic Hydroxylamine Reagent
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DOI：
10.1021/ol401290d
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发表时间：
2013-07-05
期刊：
影响因子：
5.2
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Guo, Pu;Yan, Shengyong;Zhou, Xiang
荧光羟胺试剂用于通过标记途径检测5-fC。通过化学合成、高效液相色谱、变性聚丙烯酰胺凝胶电泳、DNA质谱等方法验证了该探针与5-fC的反应具有寡核苷酸选择性，实现了荧光法对5-fC的方便定量检测。还研究了荧光检测基因组中5-fC的可行性。
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.