Golgi apparatus, GERL, and secretory granule formation within neurons of the hypothalamo-neurohypophysial system of control and hyperosmotically stressed mice.
Golgi apparatus, GERL, and secretory granule formation within neurons of the hypothalamo-neurohypophysial system of control and hyperosmotically stressed mice.
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DOI：
10.1083/jcb.90.2.474
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发表时间：
1981-08
期刊：
影响因子：
--
通讯作者：
Oliver C
中科院分区：
文献类型：
--
作者：
Broadwell RD;Oliver C
下丘脑-神经垂体系统的抗利尿激素分泌神经元是考虑高尔基体和GERL之间的关系及其在分泌颗粒产生中的作用的一个特别好的模型，因为这些神经元在高渗应激反应中增加了抗利尿激素的合成和分泌。采用酶细胞化学技术测定视上核细胞体中酸性磷酸酶（AcPase）和硫胺素焦磷酸酶（TPPase）活性，分别从正常小鼠、饮用2%盐水的高渗应激小鼠和高渗应激后恢复5-10 d的小鼠中区分GERL和高尔基体。在未孵育的对照视上核周制剂中，高尔基体反表面的未成熟分泌颗粒经常附着在一个狭窄、光滑的膜池上，该池被称为GERL。偶见分泌性颗粒附着在高尔基小囊上。TPPase活性存在于一个或两个反式高尔基小囊中；AcPase活性出现在GERL和附着的未成熟分泌颗粒中，很少出现在反式高尔基小囊和次级溶酶体中。由于高渗应激，高尔基体肥大，所有高尔基小囊和GERL形成分泌颗粒。GERL中很少或没有AcPase活性，而所有高尔基小囊和GERL样池均呈TPPase阳性。恢复期间，GERL的AcPase活性恢复正常；然而，在高渗应激下，在gerl样池和所有高尔基小囊中观察到的TPPase活性升高和分泌颗粒形成持续存在。这些结果表明，在正常情况下，GERL是分泌颗粒形成的主要部位，但在高渗应激下，高尔基体小囊在这一功能中发挥了更重要的作用。在高尔基小囊和GERL中观察到的细胞化学调节表明GERL在结构和功能上与高尔基小囊相关。
The vasopressin-producing neurons of the hypothalamo-neurohypophysial system are a particularly good model with which to consider the relationship between the Golgi apparatus nd GERL and their roles in secretory granule production because these neurons increase their synthesis and secretion of vasopressin in response to hyperosmotic stress. Enzyme cytochemical techniques for acid phosphatase (AcPase) and thiamine pyrophosphatase (TPPase) activities were used to distinguish GERL from the Golgi apparatus in cell bodies of the supraoptic nucleus from normal mice, mice hyperosmotically stressed by drinking 2% salt water, and mice allowed to recover for 5-10 d from hyperosmotic stress. In nonincubated preparations of control supraoptic perikarya, immature secretory granules at the trans face of the Golgi apparatus were frequently attached to a narrow, smooth membrane cisterna identified as GERL. Secretory granules were occasionally seen attached to Golgi saccules. TPPase activity was present in one or two of the trans Golgi saccules; AcPase activity appeared in GERL and attached immature secretory granules, rarely in the trans Golgi saccules, and in secondary lysosomes. As a result of hyperosmotic stress, the Golgi apparatus hypertrophied, and secretory granules formed from all Golgi saccules and GERL. Little or no AcPase activity could be demonstrated in GERL, whereas all Golgi saccules and GERL-like cisternae were TPPase positive. During recovery, AcPase activity in GERL returned to normal; however, the elevated TPPase activity and secretory granule formation seen in GERL-like cisternae and all Golgi saccules during hyperosmotic stress persisted. These results suggest that under normal conditions GERL is the predominant site for the secretory granule formation, but during hyperosmotic stress, the Golgi saccules assume increased importance in this function. The observed cytochemical modulations in Golgi saccules and GERL suggest that GERL is structurally and functionally related to the Golgi saccules.