Metal toxicity for rabbit alveolar macrophages in vitro.
Metal toxicity for rabbit alveolar macrophages in vitro.
复制标题
体外金属对兔肺泡巨噬细胞的毒性。
DOI：
10.1016/0013-9351(75)90047-x
复制
发表时间：
1975
影响因子：
8.3
通讯作者：
D. Coffin
中科院分区：
文献类型：
--
作者：
M. Waters;D. Gardner;C. Aranyi;D. Coffin
一个体外模型系统已被用来评估作为环境污染物的可溶性金属盐的相对细胞毒性特性。通过肺灌洗获得的兔肺泡巨噬细胞在组织培养中暴露于Cd 2+、Ni 2+、Mn 2+和Cr 3+的氯化物和钒酸铵（VO 3 −）20小时。所有金属，除镉+产生显着减少的细胞数量的浓度，影响细胞活力。Cd 2+在降低细胞活力而不引起细胞溶解方面是独特的。在比较各种金属的相对细胞毒性时，将20小时后剩余的活细胞数表示为20小时时对照培养物中细胞总数的百分比。因此，在0.1 mmCd 2+和VO 3 −或4-5 mmNi 2+、Mn 2+和Cr 3+浓度下，活细胞的净数量减少至对照的50%。酸性磷酸酶，溶酶体指示酶的比活性，也在类似的浓度下降。使用扫描电子显微镜，它是可能的相关表面变化与曝光浓度和细胞活力，从而提出一种模式和序列的细胞损伤，可能最终导致细胞死亡。
A model systemin vitrohas been employed to assess the relative cytotoxic properties of soluble salts of metals that occur as environmental contaminants. Rabbit alveolar macrophages obtained by lung lavage were exposed in tissue culture for 20 hours to chlorides of Cd2+, Ni2+, Mn2+, and Cr3+and to ammonium vanadate (VO3−). All metals except Cd2+produced significant decreases in numbers of cells at concentrations that affected cell viability. Cd2+was unique in decreasing cell viability without causing cell lysis. In comparing the relative cytotoxicity of the various metals, the number of viable cells remaining after 20 hours was expressed as a percent of the total number of cells in control cultures at 20 hours. Thus, the net number of viable cells was decreased to 50% of control at concentrations of 0.1 mmCd2+and VO3−or 4–5 mmNi2+, Mn2+, and Cr3+. The specific activity of acid phosphatase, a lysosomal indicator enzyme, was also decreased at similar concentrations. Using scanning electron microscopy it was possible to correlate surface alterations with exposure concentrations and cell viabilities so as to suggest a mode and sequence of cell injury which may ultimately lead to cell death.