Involutional Ectropion and Entropion: Clinicopathologic Correlation Between Horizontal Eyelid Laxity and Eyelid Extracellular Matrix
Involutional Ectropion and Entropion: Clinicopathologic Correlation Between Horizontal Eyelid Laxity and Eyelid Extracellular Matrix
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DOI：
10.1097/iop.0b013e31821637e4
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发表时间：
2011-09-01
影响因子：
2
通讯作者：
Belfort, Rubens, Jr.
中科院分区：
文献类型：
--
作者：
Damasceno, Renato Wendell;Osaki, Midori Hentona;Belfort, Rubens, Jr.
目的：探讨水平眼睑松弛与细胞外基质成分（如胶原和弹性纤维）在眼睑外翻和内翻中的临床病理关系。另一个目的是利用计算机辅助形态测定法比较外翻和内翻在细胞外基质含量方面的差异。方法：本临床病理研究包括20例连续的睑外翻患者（1组）和20例连续的睑内翻患者（2组）。两组均采用捏法测量眼睑水平松弛度。全层眼睑活检标本采用光镜和计算机辅助形态测定法进行检查。进行Mann-Whitney U检验、Pearson卡方检验、Pearson相关系数计算和线性回归分析。结果：1组和2组标本切片均显示胶原和弹性纤维异常。Pearson相关系数显示，水平眼睑松弛度与眼睑皮肤、睑前轮匝肌、睑板周围跗骨间质、睑板间质细胞外基质含量呈显著负相关。线性回归表明，水平眼睑松弛程度依赖于所有眼睑区域的细胞外基质成分。与2组患者标本相比，1组患者标本中胶原纤维含量显著增加。结论：目前的研究结果表明，胶原蛋白和弹性纤维的减少可能有助于下眼睑外翻和内翻患者过度水平眼睑松弛的发展。（眼科整形外科2011；27:21 1-326）
Purpose: To investigate the clinicopathologic correlation between horizontal eyelid laxity and extracellular matrix components, such as collagen and elastic fibers, in involutional ectropion and entropion. Another goal was to compare the differences between involutional ectropion and entropion in regard to extracellular matrix content using computer-assisted morphometry.Methods: This clinicopathologic study included 20 consecutive patients with involutional ectropion (group 1) and 20 consecutive patients with involutional entropion (group 2). The pinch test was performed to measure horizontal eyelid laxity in both groups. Full-thickness eyelid biopsy specimens were examined by light microscopy and computer-assisted morphometry. The Mann-Whitney U test, the Pearson chi-square test, the Pearson correlation coefficient calculation, and a linear regression analysis were performed.Results: All sections of specimens from patients in groups 1 and 2 revealed abnormal collagen and elastic fibers. The Pearson correlation coefficient revealed a significant negative correlation between horizontal eyelid laxity and extracellular matrix content in the eyelid skin, the pretarsal orbicularis oculi muscle, the perimeibomian tarsal stroma, and the intermeibomian tarsal stroma. Linear regression demonstrated that horizontal eyelid laxity is dependent upon extracellular matrix components in all eyelid regions. Collagen fiber content was significantly increased in specimens from patients in group 1 compared with specimens from patients in group 2.Conclusions: The present findings suggest that a reduction of collagen and elastic fibers may contribute to the development of excessive horizontal eyelid laxity in patients with involutional ectropion and entropion of the lower eyelid. (Ophthal Plast Reconstr Surg 2011;27:321-326)