Saccharomyces cerevisiae Pra1p/Yip3p interacts with yip1p and Rab proteins
Saccharomyces cerevisiae Pra1p/Yip3p interacts with yip1p and Rab proteins
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DOI：
10.1006/bbrc.2001.6242
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发表时间：
2002-01-18
影响因子：
3.1
通讯作者：
Collins, RN
中科院分区：
文献类型：
--
作者：
Calero, M;Collins, RN
膜运输的调节涉及Ras相关GTP酶的Rab家族，其中在酵母酿酒酵母中总共有11个成员。先前的工作已经鉴定出PRA 1是一种双异戊二烯化的Rab GTP酶和VAMP 2相互作用蛋白[Martinic等（1999）J.Biol.Chem.272，26991-26998]。在这项研究中，我们表明，酵母对应的PRA 1与Rab蛋白和Yip 1 p，膜蛋白的未知功能，已被报道，特别是与Rab蛋白Ypt 1 p和Ypt 31 p相互作用。酵母Pra 1 p/Yip 3 p是一种能够与一组不同的Rab蛋白质发生生化相互作用的因子，并且对任何特定的Rab都没有体外特异性。互动是。Tween Pra 1 p/Yip 3 p和Rab蛋白依赖于Rab蛋白C-末端半胱氨酸的存在，并需要C-末端异戊烯化。(C)2002年，Elsevier Science。
The regulation of membrane traffic involves the Rab family of Ras-related GTPases, of which there are a total of 11 members in the yeast Saccharomyces cerevisiae. Previous work has identified PRA1 as a dual prenylated Rab GTPase and VAMP2 interacting protein [Martinic et al. (1999) J. Biol. Chem. 272, 26991-26998]. In this study we demonstrate that the yeast counterpart of PRA1 interacts with Rab proteins and with Yip1p, a membrane protein of unknown function that has been reported to interact specifically with the Rab proteins Ypt1p and Ypt31p. Yeast Pra1p/Yip3p is a factor capable of biochemical interaction with a panel of different Rab proteins and does not show in vitro specificity for any particular Rab. The interactions be. tween Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. (C) 2002 Elsevier Science.