Articular cartilage proteoglycans from normal and osteoarthritic mice.
Articular cartilage proteoglycans from normal and osteoarthritic mice.
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来自正常和骨关节炎小鼠的关节软骨蛋白多糖。
DOI：
10.1002/art.1780290113
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发表时间：
1986
影响因子：
--
通讯作者：
Christner,JE
中科院分区：
文献类型：
--
作者：
Rostand,KS;Baker,JR;Caterson,B;Christner,JE
用35 S-硫酸盐在体内标记来自骨关节炎小鼠品系STR/IN的关节软骨蛋白聚糖，并对可提取性、聚集能力、单体和糖胺聚糖链的大小、糖胺聚糖的硫酸化、硫酸软骨素-4和硫酸软骨素-6的相对量以及连接蛋白进行表征。在对照组和骨关节炎动物中，0.4M盐酸胍可提取的35 S标记蛋白多糖的比例相同。然而，与对照小鼠相比，STR/IN动物中更大比例的4 M盐酸胍可提取。对照组和骨关节炎小鼠中35 S-蛋白聚糖聚集的能力相当，这是通过它们在Sepharose CL-2B上的排除来判断的。来自对照和骨关节炎动物的单体从Sepharose CL-2B洗脱，KAV为0.47。从Sepharose CL-6 B上洗脱的对照组和骨关节炎动物的糖胺聚糖的KAV为0.63，未发现硫酸化或硫酸软骨素-4含量的差异。用连接蛋白特异性抗血清免疫沉淀聚集体，在对照组和骨关节炎动物的聚集体中仅发现连接蛋白2。
Articular cartilage proteoglycans from an osteoarthritic mouse strain, STR/IN, were labeled in vivo with35S‐sulfate and characterized with respect to extractability, ability to aggregate, size of monomer and glycosaminoglycan chains, sulfation of glycosaminoglycans, relative amounts of chondroitin‐4 sulfate and chondroitin‐6 sulfate, and link proteins. The proportion of35S‐labeled proteoglycans extractable by 0.4Mguanidine hydrochloride was the same in control and osteoarthritic animals. However, a greater proportion was extractable by 4Mguanidine hydrochloride in the STR/IN animals as compared with the control mice. The ability of the35S‐proteoglycans to aggregate was comparable in controls and osteoarthritic mice, as judged by their exclusion on Sepharose CL‐2B. Monomers from both controls and osteoarthritic animals eluted from Sepharose CL‐2B with a KAVof 0.47. Glycosaminoglycans from control and osteoarthritic animals eluted from Sepharose CL‐6B with a KAVof 0.63, and no differences in sulfation or chondroitin‐4 sulfate content were found. Aggregates were immunoprecipitated with link protein‐specific antiserum, and only link protein 2 was found in aggregates from control and osteoarthritic animals.