Characterization of non-typhoidal Salmonella isolates from children with acute gastroenteritis, Kolkata, India, during 2000-2016
Characterization of non-typhoidal Salmonella isolates from children with acute gastroenteritis, Kolkata, India, during 2000-2016
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DOI：
10.1007/s42770-019-00213-z
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发表时间：
2020-01-02
影响因子：
2.2
通讯作者：
Dutta, Shanta
中科院分区：
文献类型：
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作者：
Jain, Priyanka;Chowdhury, Goutam;Dutta, Shanta
非伤寒沙门氏菌（NTS）是引起儿童急性胃肠炎的重要原因。该研究的目的是确定印度加尔各答一家医院的结核病监测中NTS的分离率、血清型流行率、抗菌素耐药性（AMR）谱和分子亚型。从2000年至2016年患有急性胃肠炎的儿童（<5岁）中收集直肠拭子。按照鉴别NTS的标准程序处理样品。对分离株进行抗菌药物敏感性、AMR基因、质粒谱、多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）亚型检测。从9957份处理样品中共回收了99株（1.0%）沙门氏菌分离株。在17个沙门氏菌血清型中，S. Worthington（33%）占优势，其次为S.肠球菌（13%）、沙门氏菌（S.鼠伤寒沙门氏菌（12%）和其他。耐药菌株对萘啶酸、氨苄西林、第三代头孢菌素、阿奇霉素耐药率分别为43%、34%、32%、25%，对氟喹诺酮类耐药率较低（2%）。产超广谱β-内酰胺酶（bla（CTX-M-15）和bla（SHV-12）基因）和阿奇霉素耐药（mphA基因）的S. Worthington，而对氟喹诺酮耐药（gyrA和parC突变）的S.肯塔基州。不同的质粒谱之间观察到的分离株。PFGE分析鉴定了循环中每个血清型的遗传相关菌株。MLST还揭示了S. Worthington ST 592和耐环丙沙星的S.肯塔基州ST 198在印度没有报告。对当前选择的药物具有耐药性的NTS构成了潜在的公共卫生问题。建议持续监测NTS血清型的AMR谱和分子亚型，以控制耐药微生物的传播。
Non-typhoidal Salmonella (NTS) is an important cause of acute gastroenteritis in children. The study was undertaken to determine the isolation rate, serovar prevalence, antimicrobial resistance (AMR) profiles, and molecular subtypes of NTS from a hospital-based diarrheal disease surveillance in Kolkata, India. Rectal swabs were collected from children (< 5 years of age) with acute gastroenteritis from 2000 to 2016. Samples were processed following standard procedures for identification of NTS. The isolates were tested for antimicrobial susceptibility, AMR genes, plasmid profiles, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE) subtypes. A total of 99 (1.0%) Salmonella isolates were recovered from 9957 samples processed. Of the 17 Salmonella serovars identified, S. Worthington (33%) was predominant followed by S. Enteritidis (13%), S. Typhimurium (12%), and others. The isolates showed high resistance towards nalidixic acid (43%), ampicillin (34%), third-generation cephalosporins (32%), and azithromycin (25%), while low resistance was observed for fluoroquinolones (2%). Extended-spectrum beta-lactamase production (bla(CTX-M-15) and bla(SHV-12) genes) and azithromycin resistance (mphA gene) were common in S. Worthington, while fluoroquinolone resistance (gyrA and parC mutations) was found in S. Kentucky. Diverse plasmid profiles were observed among the isolates. PFGE analysis identified genetically related strains of each serovar in circulation. MLST also revealed phylogenetically clonal isolates of which S. Worthington ST592 and ciprofloxacin-resistant S. Kentucky ST198 were not reported earlier from India. NTS resistant to current drugs of choice poses a potential public health problem. Continuous monitoring of AMR profiles and molecular subtypes of NTS serovars is recommended for controlling the spread of resistant organisms.