Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network
Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network
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DOI：
10.1101/gr.234503
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发表时间：
2003-02-01
期刊：
影响因子：
7
通讯作者：
Nielsen, J
中科院分区：
文献类型：
--
作者：
Förster, J;Famili, I;Nielsen, J
使用当前可用的基因组，生化和生理信息重建了酿酒酵母的酵母菌中的代谢网络。在细胞质和线粒体之间对代谢反应进行了分室，并包括隔室和环境之间的运输步骤。在重建的网络中总共考虑了708个结构性开放式阅读框（ORF），对应于1035个代谢反应。此外，基于生化证据，包括140种反应，导致基因组规模的重建代谢网络，其中包含1175个代谢反应和584个代谢产物。重建网络中包含的基因函数数量对应于酿酒酵母中所有特征性ORF的16％。使用重建的网络，计算了酿酒酵母的代谢能力，并与大肠杆菌进行了比较。重建的代谢网络是真核生物的第一个综合网络，它可以用作表型函数硅分析的基础。
The metabolic network in the yeast Saccharomyces cerevisiae was reconstructed using currently available genomic, biochemical, and physiological information. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, and transport steps between the compartments and the environment were included. A total of 708 structural open reading frames (ORFs) were accounted for in the reconstructed network, corresponding to 1035 metabolic reactions. Further, 140 reactions were included on the basis of biochemical evidence resulting in a genome-scale reconstructed metabolic network containing 1175 metabolic reactions and 584 metabolites. The number of gene functions included in the reconstructed network corresponds to similar to16% of all characterized ORFs in S. cerevisiae. Using the reconstructed network, the metabolic capabilities of S. cerevisiae were calculated and compared with Escherichia coli. The reconstructed metabolic network is the first comprehensive network for a eukaryotic organism, and it may be used as the basis for in silico analysis of phenotypic functions.