The MKK3-MPK7 cascade phosphorylates ERF4 and promotes its rapid degradation to release seed dormancy in Arabidopsis
The MKK3-MPK7 cascade phosphorylates ERF4 and promotes its rapid degradation to release seed dormancy in Arabidopsis
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DOI：
10.1016/j.molp.2023.09.006
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发表时间：
2023-11-06
期刊：
影响因子：
27.5
通讯作者：
Xiang, Yong
中科院分区：
文献类型：
--
作者：
Chen, Xi;Li, Qiujia;Xiang, Yong
种子建立休眠以延迟发芽，直到有利的生长季节到来。在这项研究中，我们确定了一个命运开关组成的MKK 3-MPK 7激酶级联和乙烯反应因子ERF 4是负责种子状态从休眠到萌发的过渡。我们发现，休眠打破因子激活MKK 3-MPK 7模块，这影响了一些α-EXPANSIN（EXPA）基因的表达，以控制种子休眠。此外，我们确定了该模块的直接下游底物ERF 4，其通过直接结合到其外显子区域中的GCC盒来抑制这些EXPA的表达。活化的MKK 3-MPK 7模块磷酸化ERF 4，导致其快速降解，从而释放其对这些EXPA表达的抑制作用。总的来说，我们的工作确定了一条由蛋白质磷酸化、降解和基因转录组成的信号链，通过该信号链，胚胎内的发芽促进剂被来自环境条件的发芽信号激活。
Seeds establish dormancy to delay germination until the arrival of a favorable growing season. In this study, we identify a fate switch comprised of the MKK3-MPK7 kinase cascade and the ethylene response factor ERF4 that is responsible for the seed state transition from dormancy to germination. We show that dormancy-breaking factors activate the MKK3-MPK7 module, which affects the expression of some a-EXPANSIN (EXPA) genes to control seed dormancy. Furthermore, we identify a direct downstream substrate of this module, ERF4, which suppresses the expression of these EXPAs by directly binding to the GCC boxes in their exon regions. The activated MKK3-MPK7 module phosphorylates ERF4, leading to its rapid degradation and thereby releasing its inhibitory effect on the expression of these EXPAs. Collectively, our work identifies a signaling chain consisting of protein phosphorylation, degradation, and gene transcription , by which the germination promoters within the embryo sense and are activated by germination signals from ambient conditions.