An unusual nucleoporin-related messenger ribonucleic acid is present in the germ cells of rat testis.
An unusual nucleoporin-related messenger ribonucleic acid is present in the germ cells of rat testis.
复制标题
大鼠睾丸的生殖细胞中存在一种不寻常的核孔蛋白相关信使核糖核酸。
DOI：
10.1095/biolreprod51.5.1022
复制
发表时间：
1994
影响因子：
3.6
通讯作者：
Kim,KH
中科院分区：
文献类型：
--
作者：
Wang,ZQ;Akmal,KM;Kim,KH
从大鼠睾丸中克隆出一种与编码核孔蛋白的大鼠p62 mRNA非常相似的mRNA。从核孔蛋白相关（NPR） cDNA的一个独特序列中提取的探针发现了一个1.3 kb的新mRNA，不同于p62基因的2.7 kb转录本。在Sertoli细胞中未检测到这个1.3 kb的转录物；它主要存在于成人睾丸的单倍体生殖细胞中。DNA测序结果显示，NPR cDNA序列的中心区域与p62 cDNA中含有七磷酸重复序列的3 '部分相同。然而，NPR cDNA序列的5′区和极3′区与p62 cDNA不同。有趣的是，极端3 '非翻译区（UTR）包含一个位于NPR cDNA中间的序列的212 bp的反向重复序列，与p62序列相同。NPR序列的反向重复可能会杂交，导致形成环状转录本。利用p62 c端特异性抗体，从NPR cDNA杂交捕获的翻译产物中检测到一个26 kda的蛋白，从睾丸生殖细胞提取物中检测到一个28 kda的蛋白，而从Sertoli细胞提取物中检测不到。
An mRNA with a substantial similarity to the rat p62 mRNA that encodes a nucleoporin was cloned from the rat testis. A probe derived from a unique sequence in the nucleoporin-related (NPR) cDNA revealed a novel mRNA of 1.3 kb, different from the 2.7-kb transcript attributed to the p62 gene. This 1.3-kb transcript was not detected in Sertoli cells; it was found primarily in the haploid germ cells of the adult testis. The DNA sequencing revealed that the central region of the NPR cDNA sequence was identical to the 3′ portion of the p6 2 cDNA containing heptad repeat sequences. However, the 5′ region and the extreme 3′ region of the NPR cDNA sequence were different from the p62 cDNA. Interestingly, the extreme 3′ untranslated region (UTR) contained a 212-bp inverted repeat of a sequence located in the middle of the NPR cDNA that is identical to the p62 sequence. The inverted repeats of the NPR sequence could potentially hybridize, leading to the formation of circular transcripts. Using antibodies specific for the C-terminal regions of p62, a 26-kDa protein was detected from NPR cDNA hybrid-arrested translational products, and a 28-kDa protein was detected from the testis germ cell extracts but not from Sertoli cell extracts.