Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress.
Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress.
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DOI：
10.3389/fpls.2017.00870
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发表时间：
2017
影响因子：
5.6
通讯作者：
Lee IJ
中科院分区：
文献类型：
--
作者：
Bilal S;Khan AL;Shahzad R;Asaf S;Kang SM;Lee IJ
本研究研究了产生植物激素的内生真菌青霉、台湾拟青霉、外生菌和Preussia sp.的除镍效率。在四种不同的内生真菌中，与铜和镉相比，台湾青霉LHL10能够耐受污染介质中高达1 mM的Ni。进一步评估了P. formosus LHL10对甘氨酸max（大豆）的修复潜力，以响应土壤Ni（0.5, 1.0和5.0 mM）的剂量依赖性增加。与未接种或未接种镍污染对照植株相比，接种台湾青松LHL10显著提高了植株生物量和生长性状。与对照相比，LHL10促进了Ni从根向茎部的转运。此外，P. formosus LHL10通过减少脂质过氧化和亚麻酸、谷胱甘肽、过氧化物酶、多酚氧化酶、过氧化氢酶和超氧化物歧化酶的积累，调节了ni污染期间大豆植株的理化机制。在Ni胁迫下，真菌接种的大豆植株的脱落酸和茉莉酸等应激响应激素显著下调。LHL10的镍修复潜力可归因于其与植物激素合成相关的基因组成。RT-PCR分析显示，产吲哚-3-乙酰胺水解酶、产吲哚-乙酸醛脱氢酶、产香叶-香叶-二磷酸合成酶、产赤霉素的烯-丁香烯氧化酶（P450-4）、产赤霉素的c13氧化酶（P450-3）均有表达。综上所述，在镍污染土壤中接种福尔摩沙能显著改善植物生长，有助于提高经济作物的植物修复能力。
This study investigated the Ni-removal efficiency of phytohormone-producing endophytic fungi Penicillium janthinellum, Paecilomyces formosus, Exophiala sp., and Preussia sp. Among four different endophytes, P. formosus LHL10 was able to tolerate up to 1 mM Ni in contaminated media as compared to copper and cadmium. P. formosus LHL10 was further assessed for its potential to enhance the phytoremediation of Glycine max (soybean) in response to dose-dependent increases in soil Ni (0.5, 1.0, and 5.0 mM). Inoculation with P. formosus LHL10 significantly increased plant biomass and growth attributes as compared to non-inoculated control plants with or without Ni contamination. LHL10 enhanced the translocation of Ni from the root to the shoot as compared to the control. In addition, P. formosus LHL10 modulated the physio-chemical apparatus of soybean plants during Ni-contamination by reducing lipid peroxidation and the accumulation of linolenic acid, glutathione, peroxidase, polyphenol oxidase, catalase, and superoxide dismutase. Stress-responsive phytohormones such as abscisic acid and jasmonic acid were significantly down-regulated in fungal-inoculated soybean plants under Ni stress. LHL10 Ni-remediation potential can be attributed to its phytohormonal synthesis related genetic makeup. RT-PCR analysis showed the expression of indole-3-acetamide hydrolase, aldehyde dehydrogenase for indole-acetic acid and geranylgeranyl-diphosphate synthase, ent-kaurene oxidase (P450-4), C13-oxidase (P450-3) for gibberellins synthesis. In conclusion, the inoculation of P. formosus can significantly improve plant growth in Ni-polluted soils, and assist in improving the phytoremediation abilities of economically important crops.