Origin and organization of the zebrafish fate map.
Origin and organization of the zebrafish fate map.
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DOI：
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发表时间：
1990-04
期刊：
影响因子：
4.6
通讯作者：
C. Kimmel;R. M. Warga;T. Schilling
中科院分区：
文献类型：
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作者：
C. Kimmel;R. M. Warga;T. Schilling
我们分析了在早期斑马鱼发育过程中通过细胞内注射示踪染料标记的细胞谱系，以了解细胞在发育过程中何时分配给特定的命运，以及命运图是如何组织的。最早的谱系限制在前面已经描述过，并且在中囊胚中将卵黄细胞与胚盘分离。在一次或两次以上的细胞分裂后，上皮包膜层（EVL）细胞的谱系变得受限于仅产生周膜。在晚期囊胚中的额外分裂之后，深层（DEL）细胞产生限于单个深层胚胎组织的克隆。EVL和DEL限制的出现可能与胚盘形态发生有关。随着DEL细胞谱系在晚期囊胚中受到限制，出现了命运图。它在组织结构上与两栖动物胚胎相似。位于早期原肠胚动物极附近的DEL细胞产生外胚层命运（包括定形表皮）。位于胚盘边缘附近的细胞产生中胚层和内胚层的命运。原肠胚中的背细胞形成胚胎的背侧和前部结构，而原肠胚中的腹侧细胞形成背侧、腹侧和后部结构。单细胞类型的祖细胞和胚胎的局部区域的确切位置不能映射在我们检查的阶段，因为在原肠胚形成过程中可变的细胞重排。
We have analyzed lineages of cells labeled by intracellular injection of tracer dye during early zebrafish development to learn when cells become allocated to particular fates during development, and how the fate map is organized. The earliest lineage restriction was described previously, and segregates the yolk cell from the blastoderm in the midblastula. After one or two more cell divisions, the lineages of epithelial enveloping layer (EVL) cells become restricted to generate exclusively periderm. Following an additional division in the late blastula, deep layer (DEL) cells generate clones that are restricted to single deep embryonic tissues. The appearance of both the EVL and DEL restrictions could be causally linked to blastoderm morphogenesis during epiboly. A fate map emerges as the DEL cell lineages become restricted in the late blastula. It is similar in organization to that of an amphibian embryo. DEL cells located near the animal pole of the early gastrula give rise to ectodermal fates (including the definitive epidermis). Cells located near the blastoderm margin give rise to mesodermal and endodermal fates. Dorsal cells in the gastrula form dorsal and anterior structures in the embryo, and ventral cells in the gastrula form dorsal, ventral and posterior structures. The exact locations of progenitors of single cell types and of local regions of the embryo cannot be mapped at the stages we examined, because of variable cell rearrangements during gastrulation.