Miniature Two-Photon Microscopic Imaging Using Dielectric Metalens.
Miniature Two-Photon Microscopic Imaging Using Dielectric Metalens.
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DOI：
10.1021/acs.nanolett.3c02439
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发表时间：
2023-08
期刊：
影响因子：
10.8
通讯作者：
Conghao Wang;Qinmiao Chen;Huilan Liu;Runlong Wu;Xiong Jiang;Qiang Fu;Zhe Zhao;Ye Zhao;Yuqian Gao;Bosong Yu;H. Jiao;Aimin Wang;Shumin Xiao;Lishuang Feng
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文献类型：
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作者：
Conghao Wang;Qinmiao Chen;Huilan Liu;Runlong Wu;Xiong Jiang;Qiang Fu;Zhe Zhao;Ye Zhao;Yuqian Gao;Bosong Yu;H. Jiao;Aimin Wang;Shumin Xiao;Lishuang Feng
微型双光子显微镜已成为研究自由活动动物大脑活动的强大技术。正在进行的研究目标包括减轻探针重量并最大限度地减少由探针附件引起的动物行为限制。采用介电超透镜可以在平面器件结构中使用相当大的光学元件，同时保持成像分辨率，是解决这些挑战的一种有前途的解决方案。在这项研究中，我们设计并制造了波长为 920 nm、高纵横比的二氧化钛超透镜。此外，还开发了重1.36克的元光学双光子显微镜。该元光学探针的横向分辨率为 0.92 μm，轴向分辨率为 18.08 μm。实验上还证明了小鼠体内大脑结构的双光子成像。平面介电超透镜技术为生物医学领域的高性能集成微型非线性显微镜和内窥镜平台提供了广阔的前景。
Miniature two-photon microscopy has emerged as a powerful technique for investigating brain activity in freely moving animals. Ongoing research objectives include reducing probe weight and minimizing animal behavior constraints caused by probe attachment. Employing dielectric metalenses, which enable the use of sizable optical components in flat device structures while maintaining imaging resolution, is a promising solution for addressing these challenges. In this study, we designed and fabricated a titanium dioxide metalens with a wavelength of 920 nm and a high aspect ratio. Furthermore, a meta-optic two-photon microscope weighing 1.36 g was developed. This meta-optic probe has a lateral resolution of 0.92 μm and an axial resolution of 18.08 μm. Experimentally, two-photon imaging of mouse brain structures in vivo was also demonstrated. The flat dielectric metalens technique holds promising opportunities for high-performance integrated miniature nonlinear microscopy and endomicroscopy platforms in the biomedical field.