Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver
Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver
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DOI：
10.1053/jhep.2000.20349
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发表时间：
2000-12-01
期刊：
影响因子：
13.5
通讯作者：
Taniguchi, H
中科院分区：
文献类型：
--
作者：
Suzuki, A;Zheng, YW;Taniguchi, H
负责组织维护和修复的干细胞存在于许多器官中。然而，肝干细胞被认为在肝脏发育和再生中起着关键作用，仍然需要很好地表征。为了解决这个问题，我们建立了一个原始肝祖细胞形成集落的培养系统。将该培养体系与荧光相结合；激活细胞分选（FACS），细胞形成集落包含不同的肝细胞和胆管细胞在胎鼠肝脏鉴定。这些细胞表达CD49f和CD29 （α 6和β 1整合素亚基），但不标记造血抗原，如CD45、TER119和c-Kit。当这些细胞移植到脾脏后，它们迁移到受体肝脏并分化为肝实质细胞。我们的研究结果表明，FAGS可以丰富肝祖细胞，并为同种异体移植替代器官和改善人工肝脏支持提供了新的途径。
Stem cells responsible for tissue maintenance and repair are found in a number of organs. However, hepatic stem cells assumed to play a key role in liver development and regeneration remain to be well characterized. To address this issue, we set up a culture system in which primitive hepatic progenitor cells formed colonies. By combining this culture system with fluorescence;activated cell sorting (FACS), cells forming colonies containing distinct hepatocytes and cholangiocytes were identified in the fetal mouse liver. These cells express both CD49f and CD29 (alpha6 and beta1 integrin subunits), but do not mark for hematopoietic antigens such as CD45, TER119, and c-Kit. When transplanted into the spleen, these cells migrated to the recipient liver and differentiated into liver parenchymal cells. Our data demonstrate that hepatic progenitor cells are enriched by FAGS and suggest approaches to supplanting organ allografting and improving artificial-organ hepatic support.