Extracorporeal Shock Wave Therapy Induces Therapeutic Lymphangiogenesis in a Rat Model of Secondary Lymphoedema
Extracorporeal Shock Wave Therapy Induces Therapeutic Lymphangiogenesis in a Rat Model of Secondary Lymphoedema
复制标题
DOI：
10.1016/j.ejvs.2011.02.029
复制
发表时间：
2011-08-01
影响因子：
5.7
通讯作者：
Satomi, S.
中科院分区：
文献类型：
--
作者：
Serizawa, F.;Ito, K.;Satomi, S.
目的：淋巴水肿是癌症治疗后常见的并发症。我们已报道低能量体外冲击波(SW)治疗可上调缺血心肌中血管内皮生长因子(VEGF)的表达。由于血管内皮生长因子在淋巴管生成中起重要作用，我们研究了我们的低能量短波疗法是否促进了大鼠淋巴管生成。方法：我们建立了大鼠淋巴水肿的尾部模型。尾部接受或不接受低能量短波治疗(0.25MJ mm(-2)，500次脉冲)4次(第3、5、7、9天)。测定吲哚青绿(ICG)的尾部体积和荧光强度。用逆转录聚合酶链式反应检测血管内皮生长因子-C和碱性成纤维细胞生长因子的表达，并用组织化学方法检测淋巴管密度。结果：对照组尾部体积明显增加，西药组明显改善。低能量短波治疗后淋巴管系统功能(ICG荧光强度)、淋巴管密度、血管内皮生长因子-C和碱性成纤维细胞生长因子的表达均增强(均P<；0.05)。结论：低能量短波治疗可通过上调血管内皮生长因子-C和碱性成纤维细胞生长因子诱导治疗性淋巴管生成，并改善大鼠尾淋巴水肿模型，提示低能量短波治疗可能是一种无创、有效的治疗淋巴水肿的方法。(C)2011年欧洲血管外科学会。爱思唯尔有限公司出版。保留所有权利。
Objective: Lymphoedema is a common complication after cancer treatment. We have reported that low-energy extracorporeal shock wave (SW) therapy up-regulates vascular endothelial growth factor (VEGF) in ischaemic myocardium. As VEGF plays an important role in lymphangiogenesis, we investigated whether our low-energy SW therapy enhances lymphangiogenesis in rats.Methods: We created a tail model of lymphoedema in rats. The tail was treated with or without low-energy SW therapy (0.25 mJ mm(-2), 500 impulses) four times (days 3, 5, 7, and 9). The tail volume and the fluorescence intensity of indocyanine green (ICG) were measured. The expression of VEGF-C and basic fibroblast growth factor (bFGF) were evaluated by RT-PCR, and the lymphatic vessel density was assessed histochemically.Results: The tail volume increased significantly in the control group and was significantly improved in the SW group. The lymphatic system function (evaluated with fluorescence intensity of ICG), the lymphatic vessel density, and the expression of VEGF-C and bFGF were all enhanced by the SW therapy (all P < 0.05).Conclusions: The low-energy SW therapy induces therapeutic lymphangiogenesis by up-regulating VEGF-C and bFGF, and improves lymphoedema in a rat-tail model, suggesting that low-energy SW therapy could be a non-invasive and effective strategy for lymphoedema in humans. (C) 2011 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.