Transcriptional profiling of the model Archaeon Halobacterium sp. NRC-1: responses to changes in salinity and temperature.
Transcriptional profiling of the model Archaeon Halobacterium sp. NRC-1: responses to changes in salinity and temperature.
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DOI：
10.1186/1746-1448-3-6
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发表时间：
2007-07-25
期刊：
影响因子：
--
通讯作者：
DasSarma S
中科院分区：
文献类型：
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作者：
Coker JA;DasSarma P;Kumar J;Müller JA;DasSarma S
模式嗜盐菌Halobacterium sp. NRC-1是第一个被完全测序的嗜盐菌，许多后基因组工具，包括全基因组DNA微阵列，现在正在应用于其分析。这种极端微生物显示出对多种压力的耐受性，包括高盐度，极端（非嗜温）温度，缺氧，紫外线和电离辐射。为了研究盐杆菌NRC-1的两个常见的压力，盐度和温度的反应，我们使用全基因组DNA微阵列分析差异生长条件下的基因表达的变化。将在42°C下在丰富培养基中有氧生长的培养物与在升高或降低的温度和高或低盐度下生长的培养物进行比较。使用定制数据库和微阵列分析工具分析获得的结果。盐胁迫条件下的生长导致许多离子转运蛋白，包括钾，磷酸盐和铁转运蛋白，以及一些肽转运蛋白和应激蛋白的基因编码的调制。生长在寒冷的温度改变了基因的表达参与脂质代谢，浮力气泡，和冷休克蛋白。热休克诱导几个已知的伴侣基因。结果表明，盐杆菌NRC-1细胞在基因表达水平上对环境变化具有高度响应性。转录谱分析表明，盐杆菌属NRC-1是高度响应其环境，并提供了一些具体的反应在基因表达水平的见解。对盐条件变化的反应似乎旨在最大限度地减少必需离子种类的损失，并减轻其他物质可能的毒性作用，而暴露于极端温度则会引起促进蛋白质折叠和限制生长抑制因子的反应。这项工作为进一步的生物信息学和遗传学研究奠定了基础，这将导致更全面地了解模式嗜盐菌的生物学。
The model halophile Halobacterium sp. NRC-1 was among the first Archaea to be completely sequenced and many post-genomic tools, including whole genome DNA microarrays are now being applied to its analysis. This extremophile displays tolerance to multiple stresses, including high salinity, extreme (non-mesophilic) temperatures, lack of oxygen, and ultraviolet and ionizing radiation. In order to study the response of Halobacterium sp. NRC-1 to two common stressors, salinity and temperature, we used whole genome DNA microarrays to assay for changes in gene expression under differential growth conditions. Cultures grown aerobically in rich medium at 42°C were compared to cultures grown at elevated or reduced temperature and high or low salinity. The results obtained were analyzed using a custom database and microarray analysis tools. Growth under salt stress conditions resulted in the modulation of genes coding for many ion transporters, including potassium, phosphate, and iron transporters, as well as some peptide transporters and stress proteins. Growth at cold temperature altered the expression of genes involved in lipid metabolism, buoyant gas vesicles, and cold shock proteins. Heat shock showed induction of several known chaperone genes. The results showed that Halobacterium sp. NRC-1 cells are highly responsive to environmental changes at the level of gene expression. Transcriptional profiling showed that Halobacterium sp. NRC-1 is highly responsive to its environment and provided insights into some of the specific responses at the level of gene expression. Responses to changes in salt conditions appear to be designed to minimize the loss of essential ionic species and abate possible toxic effects of others, while exposure to temperature extremes elicit responses to promote protein folding and limit factors responsible for growth inhibition. This work lays the foundation for further bioinformatic and genetic studies which will lead to a more comprehensive understanding of the biology of a model halophilic Archaeon.