The effect of prolonged fasting on total lipid synthesis and enzyme activities in the liver of the European eel (Anguilla anguilla).
The effect of prolonged fasting on total lipid synthesis and enzyme activities in the liver of the European eel (Anguilla anguilla).
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长期禁食对欧洲鳗鱼（Anguilla anguilla）肝脏中总脂质合成和酶活性的影响。
DOI：
10.1016/0305-0491(84)90027-0
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
F. N. Hansen
中科院分区：
文献类型：
--
作者：
S. Abraham;H. Hansen;F. N. Hansen
当鳗鱼禁食1-7周时，肝脏切片中[1- 14 C]乙酸酯的脂肪酸合成程度降低了6倍，禁食39周时降低了20倍;此后肝脏脂肪生成似乎在禁食95周时保持不变。禁食1-3周后，一些肝酶活性降低（乙酰辅酶A羧化酶降低2倍，脂肪酸合成酶降低5倍），而其他酶活性保持不变（葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、α-甘油磷酸脱氢酶以及苹果酸酶和ATP-柠檬酸裂解酶）。测定鳗鱼肝脏总脂合成和脂肪生成酶活性的最适温度为30 ℃。
The extent of fatty acid synthesis from [1-14C] acetate in liver slices was reduced 6-fold when eels were fasted for 1-7 weeks and 20-fold when fasted for 39 weeks; thereafter hepatic lipogenesis seemed to remain constant for up to 95 weeks of fasting. After a 1-3 week fast some hepatic enzyme activities were reduced (acetyl-CoA carboxylase decreased 2-fold and fatty acid synthetase declined 5-fold), while others remained unchanged (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, alpha-glycerol phosphate dehydrogenase as well as malic enzyme and ATP-citrate lyase). The optimum temperature for measuring both total lipid synthesis and lipogenic enzyme activity in eel liver was found to be 30 degrees C.