Concentrative nucleoside transporter (rCNT1) is targeted to the apical membrane through the hepatic transcytotic pathway
Concentrative nucleoside transporter (rCNT1) is targeted to the apical membrane through the hepatic transcytotic pathway
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DOI：
10.1006/excr.2002.5641
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发表时间：
2002-11-15
影响因子：
3.7
通讯作者：
Pastor-Anglada, M
中科院分区：
文献类型：
--
作者：
Duflot, S;Calvo, M;Pastor-Anglada, M
Na+依赖性核苷转运蛋白CNT 1已被确定在小窝蛋白富集质膜部分（CEF），在transcytotic内体，并在小管膜分离从静止的大鼠肝脏中的转运蛋白似乎是生物活性的。在早期/分选内体中也检测到CNT 1，尽管量很小。富含基底外侧标记物的质膜制剂显示Na+依赖性核苷转运活性，其主要（如果不是唯一的话）由CNT 2占，CNT 2是一种转运蛋白，在CEF和内体组分中均未检测到。这些数据与两种亚型在肝细胞中的不同定位和运输途径一致。CNT 1是第一个被报道遵循胞吞途径插入肝实质细胞顶侧的转运蛋白。(C)2002 Elsevier Science（美国）。
The Na+-dependent nucleoside transporter CNT1 has been identified in a caveolin-enriched plasma membrane fraction (CEF), in transcytotic endosomes, and in canalicular membranes isolated from quiescent rat liver in which the transporter appears to be biologically active. CNT1 was also detected, albeit in small amounts, in the early/sorting endosomes. Plasma membrane preparations enriched in basolateral markers showed Na+-dependent nucleoside transport activity that is mostly, if not exclusively, accounted for by CNT2, a transporter protein which was not detected in CEF nor in the endosomal fractions. These data are consistent with different localization and trafficking pathways of the two isoforms in hepatocytes. CNT1 is the first transporter which is reported to follow the transcytotic pathway to be inserted on the apical side of liver parenchymal cells. (C) 2002 Elsevier Science (USA).