Cloning and characterization of a carbohydrate metabolism-associated gene IbSnRK1 from sweetpotato
Cloning and characterization of a carbohydrate metabolism-associated gene IbSnRK1 from sweetpotato
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甘薯碳水化合物代谢相关基因 IbSnRK1 的克隆和表征
DOI：
10.1016/j.scienta.2013.04.027
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发表时间：
2013-07-04
影响因子：
4.3
通讯作者：
Liu, Qingchang
中科院分区：
文献类型：
--
作者：
Jiang, Tao;Zhai, Hong;Liu, Qingchang
蔗糖非发酵相关蛋白激酶1（SnRK 1）在植物碳水化合物代谢和淀粉合成中起重要作用。本研究从甘薯（sweetpotato，cv.鲁薯3号）的cDNA末端快速扩增（RACE）。该基因的开放阅读框（ORF）由1515个核苷酸组成，编码504个氨基酸。推导的氨基酸序列与其它植物的SnRK 1同源性较高。实时荧光定量PCR分析表明，IbSnRK 1基因在鲁薯3号叶片中的表达量显著高于茎和根。转基因烟草叶片的光合速率。与野生型相比，过量表达IbSnRK 1基因的威斯康星州38）植株中IbSnRK 1基因的表达量显著增加。蔗糖合成酶（SS）和ADP-葡萄糖焦磷酸化酶（AGG）活性分别提高11-28%和30- 92%，而蔗糖磷酸合成酶（SPS）活性降低27- 42%。蔗糖、葡萄糖、果糖和淀粉在转基因烟草植株中的积累显著高于野生型。这些结果表明，IbSnRK 1基因在甘薯糖代谢和淀粉合成中起重要作用，可用于提高甘薯可溶性糖和淀粉含量。(c)2013爱思唯尔有限公司版权所有。
Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) plays an important role in plant carbohydrate metabolism and starch biosynthesis. In the present study, a SnRK1 gene, named IbSnRK1, was isolated from sweetpotato (cv. Lushu No. 3) by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) contained 1515 nucleotides encoding 504 amino acids. The deduced amino acid sequence showed high identities with SnRK1 of other plants. Real-time quantitative PCR analysis revealed that the expression level of IbSnRK1 gene was significantly higher in leaves of Lushu No. 3 than in its stems and roots. The leaf photosynthetic rate of transgenic tobacco (cv. Wisconsin 38) plants over-expressing IbSnRK1 gene was significantly increased comparing with that of the wild-type. The activity of sucrose synthase (SS) and ADP-glucose pyrophosphorylase (AGPase) was increased by 11-28% and 30-92%, respectively, whereas the activity of sucrose phosphate synthase (SPS) was decreased by 27-42%. Sucrose, glucose, fructose and starch were found to be significantly more accumulated in transgenic tobacco plants than in the wild-type. These results suggest that IbSnRK1 gene plays important roles in carbohydrate metabolism and starch biosynthesis and may be applied for increasing soluble sugar and starch levels of sweetpotato in the future. (c) 2013 Elsevier B.V. All rights reserved.