Heavy-ion microbeam system at JAEA-Takasaki for microbeam biology
Heavy-ion microbeam system at JAEA-Takasaki for microbeam biology
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DOI：
10.1269/jrr.07085
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Kobayashi, Yasuhiko
中科院分区：
文献类型：
--
作者：
Funayama, Tornoo;Wada, Seiichi;Kobayashi, Yasuhiko
近年来，关于低剂量辐射引起的细胞反应、辐射诱导的旁观者效应和带电粒子的生物径迹结构的研究受到辐射生物学领域的特别关注。采用微束的靶照射代表了通过避免与常规照射策略相关的一些缺点来推进这项研究的有用手段。JAEA-Takasaki的重离子微束系统计划于1987年，并于1990年代初开始使用，该系统可以在大气压下使用直径最小为5 μ m的束流尺寸对生物材料进行重带电粒子的靶照射。各种生物材料已被照射使用这种微束系统，包括培养的哺乳动物和高等植物细胞，小鼠骨骼肌，蚕（家蚕）胚胎和幼虫，拟南芥根，和线虫线虫的分离纤维。该系统不仅可应用于辐射生物学领域，而且可应用于生理学、发育生物学和神经生物学等普通生物学领域的生物有机体内机制的研究，并应有助于建立和促进“微束生物学”领域。
Research concerning cellular responses to low dose irradiation, radiation-induced bystander effects, and the biological track structure of charged particles has recently received particular attention in the field of radiation biology. Target irradiation employing a microbeam represents a useful means of advancing this research by obviating some of the disadvantages associated with the conventional irradiation strategies. The heavy-ion microbeam system at JAEA-Takasaki, which was planned in 1987 and started in the early 1990's, can provide target irradiation of heavy charged particles to biological material at atmospheric pressure using a minimum beam size 5 mu m in diameter. A variety of biological material has been irradiated using this microbeam system including cultured mammalian and higher plant cells, isolated fibers of mouse skeletal muscle, silkworm (Bombyx mori) embryos and larvae, Arabidopsis thaliana roots, and the nematode Caenorhabditis elegans. The system can be applied to the investigation of mechanisms within biological organisms not only in the context of radiation biology, but also in the fields of general biology such as physiology, developmental biology and neurobiology, and should help to establish and contribute to the field of "microbeam biology".