Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study.
Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study.
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DOI：
10.1186/1749-8546-5-12
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发表时间：
2010-03-23
期刊：
影响因子：
4.9
通讯作者：
Zhu C
中科院分区：
文献类型：
--
作者：
Chen W;Dang Y;Zhu C
三七总皂苷(PNS)是三七的主要活性成分，用于治疗动脉粥样硬化、脑梗塞和脑缺血。人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1是生物活性的主要贡献者，三七总皂苷的测定对三七总皂苷的体内评价具有重要意义。本研究旨在建立同时测定人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的LC-MS/MS方法。将该方法应用于Beagle犬口服三七总皂甙后的血药动学研究。采用LC/MS/MS与固相萃取(SPE)相结合的方法。建立了同时测定Beagle犬血浆中三种主要三七总皂苷(人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1)的方法。串联质谱仪采用正离子模式下的电喷雾电离。三七皂苷r1、人参皂苷Rg1、人参皂苷Rb1的定量下限分别为0.5、0.82、1.10 ng/mL。三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的线性范围分别为2.64~264 ng/mL(R2=0.9967，P=0.003)、3.6~360 ng/mL(R2=0.9941，P=0.004)、18.7~1870 ng/mL(R2=0.9912，P=0.004)。在这些浓度范围内，三种三七总皂苷的日内和日间测定的相对标准偏差(RSD)均小于12%。将LC/MS/MS方法与固相萃取相结合，可用于三七总皂苷的药代动力学研究，如同时测定比格犬口服后血浆中总皂苷的含量。
Panax notoginseng saponins (PNS), the main active components of Radix Notoginseng, has been used for treating atherosclerosis, cerebral infarction, and cerebral ischemia. Ginsenosides Rg1, ginsenoside Rb1, and notoginsenoside R1 are the main contributors of biological activities, determination of these three saponins is very important for the in vivo evaluation of PNS. The present study aims to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of ginsenosides Rg1, ginsenoside Rb1, and notoginsenoside R1. The use of this method was exemplified in pharmacokinetic study of beagle dog plasma after oral administration of PNS. Liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was combined with solid-phase extraction (SPE). This setup was used to determine simultaneously the three major PNS (ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1) in beagle dog plasma. Tandem mass spectrometry was performed using electrospray ionization in the positive ion mode. The lower limits of quantification were 0.5 ng/mL for notoginsenoside R1, 0.82 ng/mL for ginsenoside Rg1, and 1.10 ng/mL for ginsenoside Rb1. The calibration curves for the three saponins were linear over the concentration ranges 2.64-264 ng/mL (r2 = 0.9967, P = 0.003), 3.6-360 ng/mL (r2 = 0.9941, P = 0.004), and 18.7-1870 ng/mL (r2 = 0.9912, P = 0.004) for notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1, respectively. Within these concentration ranges, the relative standard deviation (RSD) of intra- and interday assays for the three PNS from beagle dog plasma samples were less than 12%. This LC/MS/MS method in combination with SPE is useful in the pharmacokinetic study of PNS, such as the simultaneous determination of saponins in beagle dog plasma after oral administration.