Bitter taste receptors confer diverse functions to neurons
Bitter taste receptors confer diverse functions to neurons
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DOI：
10.7554/elife.11181
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发表时间：
2016-02-15
期刊：
影响因子：
7.7
通讯作者：
Carlson, John R.
中科院分区：
文献类型：
--
作者：
Delventhal, Rebecca;Carlson, John R.
苦味化合物会引起厌恶反应。在果蝇中，苦味敏感味觉神经元共同表达 Gr 味觉受体家族的许多成员。然而，苦味信号传导的分子逻辑尚不清楚。我们使用体内表达方法来分析苦味信号传导的逻辑。苦味 Grs 的异位或过度表达会增加内源性反应或赋予新的反应。令人惊讶的是，Grs 的表达也抑制了许多内源性苦味反应。相反，删除内源性 Gr 会导致新的反应。单个 Grs 的表达在不同神经元中产生显着不同的效果。结果支持苦味 Grs 相互作用的模型，表现出竞争、抑制或激活。这些结果对于味觉系统如何进化以检测新的环境危险的问题具有广泛的影响。
Bitter compounds elicit an aversive response. In Drosophila, bitter-sensitive taste neurons coexpress many members of the Gr family of taste receptors. However, the molecular logic of bitter signaling is unknown. We used an in vivo expression approach to analyze the logic of bitter taste signaling. Ectopic or overexpression of bitter Grs increased endogenous responses or conferred novel responses. Surprisingly, expression of Grs also suppressed many endogenous bitter responses. Conversely, deletion of an endogenous Gr led to novel responses. Expression of individual Grs conferred strikingly different effects in different neurons. The results support a model in which bitter Grs interact, exhibiting competition, inhibition, or activation. The results have broad implications for the problem of how taste systems evolve to detect new environmental dangers.