Effects of Cytokines, Butyrate and Dexamethasone on Serum Amyloid A and Apolipoprotein A‐I Synthesis in Human HUH‐7 Hepatoma Cells
Effects of Cytokines, Butyrate and Dexamethasone on Serum Amyloid A and Apolipoprotein A‐I Synthesis in Human HUH‐7 Hepatoma Cells
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细胞因子、丁酸盐和地塞米松对人 HUH-7 肝癌细胞血清淀粉样蛋白 A 和载脂蛋白 A-I 合成的影响
DOI：
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发表时间：
1999
影响因子：
3.7
通讯作者：
Sattler
中科院分区：
文献类型：
--
作者：
Mallé;Leonhard;Knipping;Sattler
血清淀粉样蛋白A（SAA）和载脂蛋白A-I（apo A-I）由肝脏分泌。由于两种载脂蛋白的浓度在正常和急性期条件下呈负相关，因此用白细胞介素（IL）-1 α（100和200 U）、IL-6（50和100 U）、丁酸盐（2 m m）和地塞米松（2 × 10−7 m和1 × 10−6 m）单独或联合刺激人HUH-7肝癌细胞。 在用[35 S]-甲硫氨酸代谢标记细胞后，监测SAA和apo A-I合成的变化。免疫沉淀细胞内和分泌的SAA和apo A-I，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离，并计数相应条带中的放射性。细胞内载脂蛋白水平在所有刺激（单独或联合）下分别增加2.7 - 5.5倍（SAA）和2.8 - 4.1倍（apo A-I）。以类似的方式，HUH-7细胞分泌的载脂蛋白水平增加了3.1 - 4.3倍（SAA）和1.9 - 3.3倍（apo A-I）。细胞因子、丁酸盐和/或地塞米松联合给药对SAA和apo A-I的细胞内生物合成和分泌无明显协同作用。本研究的结果表明，apo A-I不一定被视为阴性急性期反应物。
Serum amyloid A (SAA) and apolipoprotein A‐I (apo A‐I) are secreted by the liver. As concentrations of both apolipoproteins are inversely related under normal and acute‐phase conditions, human HUH‐7 hepatoma cells were stimulated with interleukin (IL)‐1α (100 and 200 U), IL‐6 (50 and 100 U), butyrate (2 m m) and dexamethasone (2 × 10−7 m and 1 × 10−6 m), alone or in combination. Changes in SAA and apo A‐I synthesis were monitored after metabolic labelling of the cells with [35S]‐methionine. Intracellular and secreted SAA and apo A‐I were immunoprecipitated, separated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE), and the radioactivity in the corresponding bands was counted. Intracellular apolipoprotein levels were increased by all stimuli, either alone or in combination, between 2.7‐ and 5.5‐fold (SAA) and between 2.8‐ and 4.1‐fold (apo A‐I), respectively. In a similar manner, apolipoprotein levels secreted by HUH‐7 cells were increased between 3.1‐ and 4.3‐fold (SAA) and between 1.9‐ and 3.3‐fold (apo A‐I). Co‐administration of cytokines, butyrate and/or dexamethasone had no pronounced synergistic effect on intracellular biosynthesis and secretion of SAA and apo A‐I. The results from the present study suggest that apo A‐I must not necessarily be considered as a negative acute‐phase reactant.
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影响因子：
6.5
作者：
K. Feingold;Ingibjorg Hardardottir;R. Memon;Eveline J. T. Krul;Arthur H. Maser;John M. Taylor;Carl Grunfeld
通讯作者：
K. Feingold;Ingibjorg Hardardottir;R. Memon;Eveline J. T. Krul;Arthur H. Maser;John M. Taylor;Carl Grunfeld
DOI：
10.1016/s0021-9258(18)34048-1
发表时间：
1982-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. S. Hoffman;E. Benditt
通讯作者：
J. S. Hoffman;E. Benditt
DOI：
10.1016/s0006-291x(88)80043-3
发表时间：
1988-11-30
影响因子：
3.1
作者：
GANAPATHI, MK;MAY, LT;KUSHNER, I
通讯作者：
KUSHNER, I
DOI：
10.1161/01.atv.14.1.8
发表时间：
1994-01-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
ETTINGER, WH;VARMA, VK;VERDERY, PB
通讯作者：
VERDERY, PB
地塞米松可增加培养基中载脂蛋白 A-I 的浓度以及 Hep G2 细胞中载脂蛋白 A-I mRNA 的丰度。
DOI：
10.1016/0026-0495(92)90288-l
发表时间：
1992
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Varma,VK;Smith,TK;Sorci-Thomas,M;EttingerJr,WH
通讯作者：
EttingerJr,WH