Malathion-induced hepatotoxicity in rats: The effects of vitamins C and E
Malathion-induced hepatotoxicity in rats: The effects of vitamins C and E
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DOI：
10.1016/j.fct.2009.11.044
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发表时间：
2010-02-01
影响因子：
4.3
通讯作者：
Kalender, Yusuf
中科院分区：
文献类型：
--
作者：
Kalender, Suna;Uzun, Fatma Gokce;Kalender, Yusuf
对成年雄性Wistar大鼠（体重300-320克，每组6只动物）给予马拉硫磷（27毫克/千克;口服剂量LD 50的1/50）。维生素C（200 mg/kg）+维生素E（200 mg/kg），或两者，每天通过管饲法持续4周。第4周末，马拉硫磷组和马拉硫磷+维生素组的白色细胞（WBC）和血小板计数均显著高于对照组。与对照组相比，两组的血清总胆固醇、碱性磷酸酶（ALP）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和乳酸脱氢酶（LDH）水平也显著升高，甘油三酯和极低密度脂蛋白（VLDL）水平显著降低。马拉硫磷处理的大鼠也具有显著较低的血清总蛋白和白蛋白水平，但马拉硫磷加维生素处理组在这些参数方面与对照组没有差异。此外，伴随的维生素处理至少部分地显著正常化，马拉硫磷改变的所有其他血液学和生物化学参数光显微镜分析显示，马拉硫磷-处理组和马拉硫磷加维生素处理组在肝组织中表现出组织病理学变化，尽管仅在马拉硫磷处理组中观察到一些病理学特征。因此，维生素C和E可以降低马拉硫磷的肝毒性，尽管它们提供的保护程度有限。(C)2009爱思唯尔有限公司版权所有
Mature male Wistar rats (weighing 300-320 g and each group six animals) were given malathion (27 mg/kg; 1/50 of the LD50 for an oral dose). vitamin C (200 mg/kg) + vitamin E (200 mg/kg), or both daily via gavage for 4 weeks. At the end of the fourth week, the malathion-treated group and the malathion plus vitamin-treated group both had significantly higher white blood cell (WBC) and thrombocyte Counts than the control group. Compared to the control group, the two groups also had significantly higher serum total cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels, and significantly lower triglyceride and very low density lipoprotein (VLDL) levels. The malathion-treated rats also had significantly lower serum total protein and albumin levels, but the malathion plus vitamin-treated group did not differ from the control group in terms of these parameters Moreover, concomitant vitamin treatment significantly normalized, at least partially, all of the other hematological and biochemical parameters that were altered by malathion Light microscopic analyses revealed that both the malathion-treated and malathion plus vitamin-treated groups exhibited histopathological changes in liver tissues, although some pathological features were only observed in the malathion-treated group. Thus, vitamins C and E can reduce malathion hepatotoxicity, although the degree of protection they provide is limited. (C) 2009 Elsevier Ltd. All rights reserved