Novel non-enzymatic toxic peptide of Daboia russelii (Eastern region) venom renders commercial polyvalent antivenom ineffective
Novel non-enzymatic toxic peptide of Daboia russelii (Eastern region) venom renders commercial polyvalent antivenom ineffective
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DOI：
10.1016/j.toxicon.2005.12.002
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发表时间：
2006-03-15
期刊：
影响因子：
2.8
通讯作者：
Gowda, TV
中科院分区：
文献类型：
--
作者：
Kumar, AVM;Gowda, TV
蛇毒液通常是酶和非酶肽的复杂混合物。从印度三个地区，罗素的毒蛇毒液的非酶促部分的区域变化进行了研究。 Sephadex-G-75色谱柱上的凝胶渗透色谱的东部，西部和南部毒液分为三个峰。所有三个重叠峰的致死性和酶促效力都不同。所有区域毒液的峰值III被发现是非酶，西部和南部毒液具有胰蛋白酶抑制活性，具有不同的效力。有趣的是，东部地区的峰III没有胰蛋白酶抑制活性。但是它具有高度致死的，LD50 0.7 mg/kg体重，并且表现出突触后神经毒性。另一方面，南部和西部区域毒液的非酶峰是非蛋白质的，并且在实验模型中不会诱导神经毒性症状。与其他区域毒液的峰I和II交叉反应的东部区域毒液发生的抗体，但未与西方和南部地区罗素的毒蛇毒液的III峰进行过反应。准备中和印度常见毒蛇的毒性作用的商业抗毒液对所有三种毒液测试的峰值I，II和III的毒性作用呈阳性，除了东部区域毒液的峰III以外。商业抗毒液中和西部和南部地区罗素的毒蛇毒液的致命毒性，未能中和东部地区罗素的毒蛇毒液的致命作用。 （c）2005 Elsevier Ltd.保留所有权利。
The snake venoms are typically complex mixtures of enzymes and non-enzymatic peptides. Regional variation in the nonenzymatic fraction of Russell's viper venom from three regions of India Studied. The eastern, western and southern regional venom upon gel permeation chromatography on sephadex-G-75 column resolved into three peaks. All the three overlapping peaks differ in their lethality and enzymatic potency. Peak III of all the regional venom found to be non-enzymatic, Western and southern regional venom has trypsin inhibitory activity with varying potencies. Interestingly, the peak III of eastern region is devoid of trypsin inhibitory activity. But it is highly lethal with a LD50 0.7 mg/kg body weight and also it exhibited postsynaptic neurotoxicity. On the other hand southern and western regional venom's non-enzymatic peak is non-lethal and did not induce neurotoxic symptoms in experimental model. The antibodies developed against the eastern regional venom cross-reacted with the peaks I and II of other regional venom, but failed to cross-react with the peak III of western and southern regional Russell's viper venom. Commercial anti-venom prepared to neutralize the toxic effects of common poisonous snakes of India, showed positive cross-reaction against peaks I, II and III of all three regional venom tested, except peak III of eastern regional venom. Commercial anti-venom neutralized the lethal toxicity of both western and southern regional Russell's viper venom, and failed to neutralize the lethal effects of eastern regional Russell's viper venom. (c) 2005 Elsevier Ltd. All rights reserved.