Hydrophobic match between cholesterol and saturated fatty acid is required for the formation of lamellar liquid ordered phases
Hydrophobic match between cholesterol and saturated fatty acid is required for the formation of lamellar liquid ordered phases
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DOI：
10.1021/la0491293
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发表时间：
2004-08-31
期刊：
影响因子：
3.9
通讯作者：
Lafleur, M
中科院分区：
文献类型：
--
作者：
Ouimet, J;Lafleur, M
棕榈酸和胆固醇在一定条件下会在液体有序相（lo）中形成双分子层。在本工作中，研究了胆固醇（chol）和饱和脂肪酸（FA）的酰基链之间的疏水匹配的贡献。采用红外、H-2核磁共振光谱和差示扫描量热法研究了FA酰基链长在12 ~ 24个碳原子之间的FA/chol混合物的行为。研究发现，只有酰基链为14-18个碳原子的脂肪酸才能形成低相双分子层。事实上，这些链的长度与胆固醇分子长轴的长度相对应。因此，分子组分的极性部分之间的疏水匹配似乎是层状相形成的必要条件。
Palmitic acid and cholesterol have been shown to form, under certain conditions, bilayers in the liquid ordered (lo) phase. In the present work, the contribution of the hydrophobic match between cholesterol (chol), and the acyl chain of saturated fatty acids (FA) has been examined. The behavior of FA/chol mixtures where the FA acyl chain length was varied between 12 and 24 carbon atoms was investigated by infrared and H-2 NMR spectroscopy, as well as by differential scanning calorimetry. It was found that only fatty acids with acyl chains of 14-18 carbon atoms lead to the formation of lo phase bilayers. The length of these chains corresponds, in fact, to the length of the long axis of the cholesterol molecule. Therefore, the hydrophobic match between the apolar parts of the molecular constituents appears to be a requisite for the formation of lamellar lo phases.