Naive T-cell receptor transgenic T cells help memory B cells produce antibody
Naive T-cell receptor transgenic T cells help memory B cells produce antibody
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DOI：
10.1111/j.1365-2567.2006.02446.x
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发表时间：
2006-11-01
期刊：
影响因子：
6.4
通讯作者：
Bell, Eric B.
中科院分区：
文献类型：
--
作者：
Duffy, Darragh;Yang, Chun-Ping;Bell, Eric B.
初次免疫后注射相同抗原诱导典型的二次应答，其特征在于比初始应答更快地产生大量免疫球蛋白G类的高亲和力抗体-记忆B细胞的产物在性质上不同于原始幼稚B淋巴细胞的产物。人们对刺激记忆B细胞的CD 4 T细胞所提供的帮助知之甚少。使用抗原特异性T细胞受体转基因CD 4 T细胞（DO11.10）作为帮助来源，我们发现初始转基因T细胞刺激记忆B细胞几乎与最近启动的转基因T细胞一样（在数量和速度方面）。血清抗体水平与转移的幼稚转基因T细胞数量之间存在直接相关性。使用转基因白细胞介素-2缺陷小鼠的T细胞，我们表明，白细胞介素-2是不需要的继发性反应，虽然它是必要的主要反应。结果表明，由CD 4 T细胞传递的信号和记忆B细胞激活所需的信号对于抗原致敏的和幼稚的CD 4 T细胞是共同的。
Injection of the same antigen following primary immunization induces a classic secondary response characterized by a large quantity of high-affinity antibody of an immunoglobulin G class produced more rapidly than in the initial response - the products of memory B cells are qualitatively distinct from that of the original naive B lymphocytes. Very little is known of the help provided by the CD4 T cells that stimulate memory B cells. Using antigen-specific T-cell receptor transgenic CD4 T cells (DO11.10) as a source of help, we found that naive transgenic T cells stimulated memory B cells almost as well (in terms of quantity and speed) as transgenic T cells that had been recently primed. There was a direct correlation between serum antibody levels and the number of naive transgenic T cells transferred. Using T cells from transgenic interleukin-2-deficient mice we showed that interleukin-2 was not required for a secondary response, although it was necessary for a primary response. The results suggested that the signals delivered by CD4 T cells and required by memory B cells for their activation were common to both antigen-primed and naive CD4 T cells.