RNA required for import of precursor proteins into mitochondria.
RNA required for import of precursor proteins into mitochondria.
复制标题
将前体蛋白输入线粒体所需的 RNA。
DOI：
10.1126/science.6209799
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Rosenberg,LE
中科院分区：
文献类型：
--
作者：
Firgaira,FA;Hendrick,JP;Kalousek,F;Kraus,JP;Rosenberg,LE
细胞质RNA部分是必需的翻译后摄取的核编码的哺乳动物蛋白质的线粒体基质。核糖核酸酶的翻译后添加到网织红细胞裂解物编程的无细胞翻译混合物抑制随后的进口六种不同的线粒体基质酶前体到大鼠肝线粒体。所需的RNA受到高度保护，如产生这种抑制所必需的高浓度核糖核酸酶所示。抑制作用对温度、暴露于核糖核酸酶的持续时间和二价阳离子的可用性的依赖性是核糖核蛋白的核酸酶敏感性的特征。核糖核酸酶敏感的成分被发现在一个400千道尔顿的馏分，其中包含线粒体蛋白质的前体。
A cytoplasmic RNA moiety is necessary for posttranslational uptake of nuclear-encoded mammalian proteins destined for the mitochondrial matrix. Post-translational addition of ribonuclease to a reticulocyte lysate-programmed cell-free translation mixture inhibited subsequent import of six different mitochondrial matrix enzyme precursors into rat liver mitochondria. The required RNA is highly protected, as indicated by the high concentrations of ribonuclease necessary to produce this inhibition. The dependence of the inhibitory effect on temperature, duration of exposure to ribonuclease, and availability of divalent cations is characteristic of the nuclease susceptibility of ribonucleoproteins. The ribonuclease-sensitive component was found in a 400-kilodalton fraction which contains the mitochondrial protein precursors.