Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell
Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell
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DOI：
10.1264/jsme2.me09108
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Watanabe, Kazuya
中科院分区：
文献类型：
--
作者：
Shimoyama, Takefumi;Yamazawa, Akira;Watanabe, Kazuya
分析了在处理淀粉、蛋白胨和鱼提取物的盒式电极微生物燃料电池中开发的浮游细菌群落和阳极生物膜细菌群落之间的定量和定性差异。基于蛋白质含量和 rRNA 基因拷贝数的定量分析表明，浮游微生物的丰度是阳极生物膜微生物的八倍以上。 PCR 扩增的 16S rRNA 基因片段的克隆文库分析揭示了这两个群落中存在属于拟杆菌门、厚壁菌门和变形菌门的细菌。最丰富的序列属于紫单胞菌科，分别占浮游微生物库和生物膜微生物库中所有序列的 50% 和 20% 以上。
Quantitative and qualitative differences were analyzed between planktonic and anode-biofilm bacterial communities developed in a cassette-electrode microbial fuel cell treating starch, peptone, and fish extract. Quantitative analyses based on protein contents and rRNA-gene copy numbers indicated that planktonic microbes were over eight-times more abundant than anode-biofilm microbes. Clone-library analyses of PCR-amplified 16S rRNA gene fragments revealed the presence of bacteria affiliated with the phyla Bacteroidetes, Firmicutes, and Proteobacteria in these two communities. The most abundant sequence was affiliated with the family Porphyromonadaceae, and accounted for over 50% and 20% of all the sequences in the planktonic-and biofilm-microbe libraries, respectively.