THE EARLY CELLULAR AND HUMORAL IMMUNE-RESPONSE TO PRIMARY AND BOOSTER ORAL IMMUNIZATION WITH CHOLERA TOXIN-B SUBUNIT
THE EARLY CELLULAR AND HUMORAL IMMUNE-RESPONSE TO PRIMARY AND BOOSTER ORAL IMMUNIZATION WITH CHOLERA TOXIN-B SUBUNIT
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DOI：
10.1002/eji.1830210917
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发表时间：
1991-09-01
影响因子：
5.4
通讯作者：
GRIFFIN, GE
中科院分区：
文献类型：
--
作者：
LEWIS, DJM;NOVOTNY, P;GRIFFIN, GE
13名志愿者口服霍乱毒素B亚单位后，观察了免疫应答。 观察到血清IgG和伊加抗毒素应答，其通过第二次免疫加强。 使用免疫斑点试验，细胞自发分泌抗毒素IgG和伊加，但不是IgM出现在免疫后的血液中短暂的。 第一次免疫后7天，每2 × 10（6）个单核细胞中有105个IgG和87个IgA分泌细胞，第二次免疫后5天，每2 × 10（6）个单核细胞中有282个IgG和413个IgA分泌细胞。 观察到总IgM分泌细胞的多克隆增加。 在循环细胞反应的峰值时，在骨髓中观察到很少的抗毒素分泌细胞，这可以通过外周血污染样品来解释，表明骨髓不是口服免疫后抗毒素分泌细胞的重要部位。
The immune response to cholera toxin B subunit given orally was studied in 13 human volunteers. A serum IgG and IgA antitoxin response was observed, which was boosted by a second immunization. Using an immunospot assay, cells spontaneously secreting anti-toxin IgG and IgA, but not IgM appeared transiently in the blood after immunization. There were 105 IgG- and 87 IgA-secreting cells per 2 x 10(6) mononuclear cells 7 days after the first immunization, and 282 IgG- and 413 IgA-secreting cells 5 days after the second immunization. A polyclonal increase in total IgM-secreting cells was observed. Few anti-toxin-secreting cells were observed in the bone marrow at the peak of the circulating cell response, which could be accounted for by contamination of the sample with peripheral blood, suggesting that the bone marrow is not a significant site of anti-toxin-secreting cells after oral immunization.