Wheat zinc finger protein TaLSD1, a negative regulator of programmed cell death, is involved in wheat resistance against stripe rust fungus
Wheat zinc finger protein TaLSD1, a negative regulator of programmed cell death, is involved in wheat resistance against stripe rust fungus
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小麦锌指蛋白 TaLSD1 是程序性细胞死亡的负调节因子，参与小麦对条锈菌的抗性
DOI：
10.1016/j.plaphy.2013.07.009
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发表时间：
2013-10-01
影响因子：
6.5
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
--
作者：
Guo, Jun;Bai, Pengfei;Kang, Zhensheng
拟南芥拟病斑突变体（Isd 1）的遗传特性研究揭示了AtLSD 1在细胞死亡和抗病性负调控中的重要作用。AtI5D1中的三个锌指基序揭示了一个新的植物特异性基因家族，其成员与程序性细胞死亡（PCD）密切相关。在这项研究中，我们的特点是功能同源AtLSD 1，TaLSD 1，在小麦条锈菌致病系统。TaLSD 1在小麦与条锈菌（Puccinia striiformis f.）（Pat）的表达，并通过甲基紫精（MV）产生的氧化应激而上调。TaLSD 1主要定位于洋葱表皮细胞的细胞核中。本氏烟草中的瞬时过表达试验表明，TaLSD 1部分抑制小鼠Bax蛋白引发的程序性细胞死亡，而TaLSD 1单独表达对烟草的表型没有影响。通过病毒诱导的基因沉默（VIGS）敲低TaLSD 1的表达，小麦对Pst的抗性增强，同时伴随着过敏反应（HR）增强、PR1基因表达增加和Pst菌丝生长减少。这些结果表明，TaLSD 1基因负调控植物过敏性细胞死亡，并参与小麦对条锈病病原菌的抗病性。(C)2013年Elsevier Masson SAS。All rights reserved.
Genetic characterization of the Arabidopsis lesion simulating disease 1 (Isd1) mutant, a lesion mimic mutant (LMM), has revealed the essential role of AtLSD1 in the negative regulation of cell death and disease resistance. The three zinc-finger motifs found in AtI5D1 revealed a novel plant-specific gene family, whose members are significantly related to programmed cell death (PCD). In this study, we characterized a functional homologue to AtLSD1, TaLSD1, in the wheat-stripe rust fungus pathosystem. The expression of TaLSD1 was differentially induced during incompatible and compatible interactions between wheat and Puccinia striiformis f. sp. tritici (Pat) and was up-regulated by oxidative stress generated by methyl viologen (MV). TaLSD1 was found to be predominately localized in the nucleus of onion epidermal cell. Transient overexpression assays in Nicotiana benthamiana demonstrated that TaLSD1 partially inhibited programmed cell death triggered by a mouse Bax protein, whereas expression of TaLSD1 alone had no influence on the phenotype of tobacco. Knocking down the expression of TaLSD1 through virus-induced gene silencing (VIGS) increased wheat resistance against Pst accompanied by an enhanced hypersensitive response (HR), an increase in PR1 gene expression and a reduction in Pst hyphal growth. Our results suggest that TaLSD1 functions negatively in regulating the plant hypersensitive cell death and is involved in disease resistance of wheat against the stripe rust pathogen. (C) 2013 Elsevier Masson SAS. All rights reserved.