Plant lectin-like antibacterial proteins from phytopathogens Pseudomonas syringae and Xanthomonas citri
Plant lectin-like antibacterial proteins from phytopathogens Pseudomonas syringae and Xanthomonas citri
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DOI：
10.1111/j.1758-2229.2012.00331.x
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发表时间：
2012-08-01
影响因子：
3.3
通讯作者：
De Mot, Rene
中科院分区：
文献类型：
--
作者：
Ghequire, Maarten G. K.;Li, Wen;De Mot, Rene
丁香假单胞菌的基因组。丁香科642和柑橘黄单胞菌。malvacearum LMG 761每个都携带假定的同源植物凝集素样细菌素（llpA）基因，这些基因先前在根际分离的恶臭假单胞菌BW11M1和生物防治菌株荧光假单胞菌Pf-5中发现。分别纯化的重组蛋白LlpAPss642和LlpAXcm761显示出跨物种的属特异性抗菌活性。丁香假单胞菌的抑菌谱与恶臭假单胞菌和荧光假单胞菌的抑菌谱部分重叠。值得注意的是，轴足黄单胞菌pv。citri str. 306分泌与LlpAXcm761相同的蛋白。两种不同植物病原菌LlpA蛋白的功能表征-变形菌种将凝集素类细菌素家族扩展到假单胞菌属之外，并表明它参与了具有不同生活方式的密切相关的植物相关细菌之间的竞争。
The genomes of Pseudomonas syringae pv. syringae 642 and Xanthomonas citri pv. malvacearum LMG 761 each carry a putative homologue of the plant lectin-like bacteriocin (llpA) genes previously identified in the rhizosphere isolate Pseudomonas putida BW11M1 and the biocontrol strain Pseudomonas fluorescens Pf-5. The respective purified recombinant proteins, LlpAPss642 and LlpAXcm761, display genus-specific antibacterial activity across species boundaries. The inhibitory spectrum of the P. syringae bacteriocin overlaps partially with those of the P. putida and P. fluorescens LlpAs. Notably, Xanthomonas axonopodis pv. citri str. 306 secretes a protein identical to LlpAXcm761. The functional characterization of LlpA proteins from two different phytopathogenic ?-proteobacterial species expands the lectin-like bacteriocin family beyond the Pseudomonas genus and suggests its involvement in competition among closely related plant-associated bacteria with different lifestyles.