Oral microflora and their relation to risk factors in HIV+ patients with oropharyngeal candidiasis
Oral microflora and their relation to risk factors in HIV+ patients with oropharyngeal candidiasis
复制标题
DOI：
10.1016/j.mycmed.2013.02.001
复制
发表时间：
2013-06-01
影响因子：
3.6
通讯作者：
Tamami, I. Ashrafi
中科院分区：
文献类型：
--
作者：
Sharifzadeh, A.;Khosravi, A. R.;Tamami, I. Ashrafi
Objective. - 本研究的目的是确定口腔微生物菌群的患病率和口腔念珠菌病与HIV+患者多种危险因素的关系。- 本研究纳入了100例在伊朗德黑兰伊玛目霍梅尼医院接受口咽念珠菌病（OPC）和HIV治疗的HIV感染患者。我们评估OPC的存在或不存在，并从口腔中获得样本，并在所有患者中进行直接显微镜检查、革兰氏染色和标准微生物培养基培养。计算CD 4+细胞计数/CD 4+细胞百分比。- 人口统计学特征显示，患者平均年龄为32.3岁，78%为男性，22%为女性。O+血型的患者（27%）更容易发生OPC。共获得460个细菌菌落，变形链球菌（15.4%）是HIV+患者中最常见的细菌，其次是表皮葡萄球菌（12.8%）和棒状杆菌（8.7%）。此外，254酵母菌（来自四个不同的属）被分离出的患者进行研究。念珠菌属（94.4%）是最常见的属，其次是酵母属（2.4%）、克鲁维酵母属和隐球菌属（均为1.6%）。HIV阳性OPC患者念珠菌中以白色念珠菌最常见（37.2%），明显高于其他念珠菌（P < 0.05）。光滑假丝酵母（Candida glabrata）、C. dubliniensis、C. tropicalis、热带假丝酵母C. parapsilosis，C. krusei，克鲁塞伊C. lusitaniae，C. guilliermondii和C.还鉴定了norvegensis。40%的患者有口角炎作为最常见的临床变异。平均CD 4+细胞计数为154.5个细胞/μ L，范围为8 - 611个细胞/μ L。30%的患者的CD 4+细胞计数在101至200个细胞/μ L之间（占分离的总酵母菌的28.7%）。不同CD 4+细胞计数水平的HIV阳性患者亚组间酵母菌和细菌计数差异无统计学意义。- 我们的研究结果表明，念珠菌属的酵母菌分离在一个可比的速度从口腔的HIV+患者，有没有变量的CD 4+细胞计数和酵母菌计数的显着差异。这项研究的结果将有助于任何进一步的研究，如果在一个大的队列中进行前瞻性研究，可能是证实性的。(C)2013年由Elsevier Masson SAS出版。
Objective. - The purpose of this study was to determine the prevalence of oral microflora and association of oral candidiasis and multiple risk factors in HIV+ patients.Patients and methods. - The present study included 100 HIV-infected patients participated in Imam Khomeini Hospital, Tehran, Iran for Oropharyngeal candidiasis (OPC) and HIV. We assessed the presence or absence of OPC, and samples were obtained from the oral cavity and direct microscopic examination, gram staining and culture on standard microbiological media were performed in all patients. CD4+ cell count/CD4+ percentage were also calculated.Results. - The demographic characteristics showed that the patients had a mean age of 32.3 years old, 78% male and 22% female. Patients belonging to 'O+' blood group (27%) were more prone to develop OPC. A total of 460 bacterial colonies were obtained and Streptococcus mutans (15.4%) was the most frequently isolated species in the HIV+ patients, followed by Staphylococcus epidermidis (12.8%) and Corynebacterium (8.7%). In addition, 254 yeasts (from four different genera) were isolated from the patient under study. Candida species (94.4%) were the most frequently obtained genera, followed by Saccharomyces (2.4%), Kluyveromyces and Cryptococcus (1.6% for both) species. Candida albicans (37.2%) was the most common species isolated from HIV+ patients with OPC and its frequency was significantly higher than that of other Candida species (P < 0.05). Candida glabrata, C. dubliniensis, C. tropicalis, C. parapsilosis, C. krusei, C. lusitaniae, C. guilliermondii and C. norvegensis were also identified. Forty percent of the patients had angular cheilitis as the most frequent clinical variant. The mean CD4+ cell counts were 154.5 cells/mu L, with a range of 8 to 611 cells/mu L. Thirty percent patients had a CD4+ cell count between 101 and 200 cells/mu L (28.7% of total yeasts isolated). Yeast and bacteria counts did not differ statistically among HIV+ patients' subgroups with different levels of CD4+ cells counts.Conclusion. - Our results showed that yeasts of the genus Candida were isolated at a comparable rate from the oral cavity of HIV+ patients and there was no significant difference of the variables CD4+ cell count and yeast counts. The findings of this study would be helpful in any further study, which, if done prospectively on a large cohort, can be confirmatory. (C) 2013 Published by Elsevier Masson SAS.